Kuzumaki N
Gan To Kagaku Ryoho. 1987 Jun;14(6 Pt 2):2205-11.
Four mouse hybridoma cell lines rp12, rp28, rp35 and rp38 producing monoclonal antibodies (MoAbs) reactive with ras oncogene product p21 were established with the use of recombinant proteins as immunogens. Using immunofluorescence, avidin-biotin complex or peroxidase-antiperoxidase methods, the reactivity of rp12, rp28 and rp35 MoAbs was examined in fresh and paraformaldehyde- or formalin fixed tissues of malignant and benign lesions of the skin, lung, stomach, uterus and ovary. These MoAbs strongly reacted with most malignant melanomas, lung cancers, stomach cancers, colon cancers and adenomatous polyps, uterus cancers, and ovary cancers, while they weakly reacted with inflammatory lung tissues, stomach polyps and metaplastic tissues and cervical dysplastic lesions, and did not react with pigmented nevi. The cancers and colon adenomatous polyps showed a high positive cell ratio, with not only cytoplasmic but also membranous localization of the staining, while other tissues had a low positive cell ratio and cytoplasmic staining localization. The MoAbs rp12, rp28 and rp35 could therefore be helpful for differential diagnosis between cancers and benign lesions.
利用重组蛋白作为免疫原，建立了4株产生与ras癌基因产物p21反应的单克隆抗体（MoAb）的小鼠杂交瘤细胞系rp12、rp28、rp35和rp38。采用免疫荧光、抗生物素蛋白-生物素复合物或过氧化物酶-抗过氧化物酶方法，在皮肤、肺、胃、子宫和卵巢的恶性和良性病变的新鲜组织以及经多聚甲醛或福尔马林固定的组织中检测rp12、rp28和rp35单克隆抗体的反应性。这些单克隆抗体与大多数恶性黑色素瘤、肺癌、胃癌、结肠癌和腺瘤性息肉、子宫癌和卵巢癌发生强烈反应，而与炎症性肺组织、胃息肉和化生组织以及宫颈发育异常病变反应较弱，与色素痣无反应。癌症和结肠腺瘤性息肉显示出高阳性细胞率，染色不仅定位于细胞质，也定位于细胞膜，而其他组织阳性细胞率低且染色定位于细胞质。因此，单克隆抗体rp12、rp28和rp35有助于癌症与良性病变的鉴别诊断。
