Institute of Experimental Morphology, Pathology and Anthropology with Museum, Bulgarian Academy of Sciences, Acad. Georgi Bonchev Str., Bl. 25, 1113, Sofia, Bulgaria.
Department of Biomedical Imaging and Image-Guided Therapy, Division of Molecular and Structural Preclinical Imaging, Medical University of Vienna and General Hospital of Vienna, Waehringer Guertel 18-20, 1090, Vienna, Austria.
Environ Sci Pollut Res Int. 2021 Feb;28(6):6784-6795. doi: 10.1007/s11356-020-10960-4. Epub 2020 Oct 2.
In this study, we compare the effects of deferiprone (Def) and tetraethylammonium salt of salinomycinic acid (Sal) on lead (Pb)-induced toxicity in testes of Pb-exposed mice. Mature male ICR mice were allocated into four groups as follows: untreated control mice (ctrl)-received distilled water for 4 weeks; Pb-exposed mice (Pb)-subjected to 14-day Pb (II) nitrate administration at dose 80 mg/kg body weight (b.w.); Pb + Def group-Pb-exposed mice, treated with 20 mg/kg b.w. Def for 2 weeks; and Pb + Sal group-Pb-intoxicated mice, treated with 16 mg/kg b.w. Sal for 14 days. The results demonstrated that Pb exposure significantly increased blood and testicular Pb concentrations, decreased testicular calcium (Ca) content, significantly elevated testicular levels of magnesium (Mg), zinc (Zn), and selenium (Se) but did not significantly affect the endogenous contents of phosphorous (P) and iron (Fe) compared with untreated controls. Pb intoxication induced disorganization of the seminiferous epithelium. Def or Sal administration reduced blood Pb and testicular Pb concentrations in Pb-exposed mice compared with the Pb-intoxicated group. Mg, Zn, and Se concentrations in testes of Pb-exposed mice, treated with Def or Sal, remained higher compared with the untreated controls. Sal significantly increased testicular P concentration compared with untreated controls and significantly elevated the testicular Ca and Fe concentrations compared with the toxic control group. Both chelating agents improved testicular morphology to a great extent. The results demonstrate the potential of both compounds as antidotes for treatment of Pb-induced impairment of male reproductive function.
在这项研究中，我们比较了去铁酮（Def）和硫酸黏菌素四乙铵盐（Sal）对铅暴露小鼠睾丸中铅诱导毒性的影响。成熟雄性 ICR 小鼠被分为四组：未处理对照组（ctrl）-接受蒸馏水 4 周；铅暴露组（Pb）-给予 80mg/kg 体重（b.w.）的硝酸铅（II）14 天；Pb+Def 组-Pb 暴露小鼠，用 20mg/kg b.w. Def 治疗 2 周；Pb+Sal 组-Pb 中毒小鼠，用 16mg/kg b.w. Sal 治疗 14 天。结果表明，与未处理对照组相比，铅暴露显著增加了血液和睾丸中的铅浓度，降低了睾丸中的钙（Ca）含量，显著增加了睾丸中的镁（Mg）、锌（Zn）和硒（Se）水平，但对内源性磷（P）和铁（Fe）含量没有显著影响。铅中毒导致生精上皮紊乱。与铅中毒组相比，Def 或 Sal 给药降低了铅暴露小鼠的血液和睾丸中的铅浓度。用 Def 或 Sal 处理的铅暴露小鼠的睾丸中的 Mg、Zn 和 Se 浓度仍高于未处理的对照组。Sal 显著增加了睾丸中的 P 浓度，与未处理对照组相比，与毒性对照组相比，睾丸 Ca 和 Fe 浓度显著升高。两种螯合剂在很大程度上改善了睾丸形态。结果表明，这两种化合物都有作为治疗铅诱导的男性生殖功能障碍的解毒剂的潜力。
