Departament Food Technology, Research Group 'Grupo Calidad y Seguridad Alimentaria', Escuela Politécnica Superior de Orihuela (EPSO), Universidad Miguel Hernández de Elche (UMH), Alicante, Spain.
Department of Fruit, Vegetable and Plant Nutraceutical Technology, Wrocław University of Environmental and Life Sciences (UPWr), Wrocław, Poland.
J Sci Food Agric. 2021 Apr;101(6):2294-2303. doi: 10.1002/jsfa.10850. Epub 2020 Oct 22.
Obesity and type 2 diabetes mellitus are the most extended current chronic diseases and also Alzheimer pathology which is a progressive degenerative neurological disorder. Therefore, finding effective enzyme inhibitors responsible for the development of these diseases are essential. Therefore, the aim of this study was to investigate the impact of fruit purée (Cydonia oblonga, Ziziphus jujube and Malus domestica) and pomegranate juice cultivar ('Mollar de Elche' and 'Wonderful') of dried pomegranate sheets (DS) on the inhibition of enzymes associated with metabolic (α-amylase, α-glucosidase, and pancreatic lipase activity), and neurological disorder (acetylcholinesterase and butyrylcholinesterase activity). Quality properties (colour coordinates, texture properties and sensory characteristics) of DS were also studied. In addition, it was researched the effect of storage conditions (4 months at 4 and 20 °C) on phenolic content.
DS from jujube had the highest antioxidant capacity and were characterized by the highest storage stability with respect to phenolic compounds. The α-amylase and α-glucosidase half maximal inhibitory concentration (IC , in mg mL ) inhibition of DS ranged from 107 to 216 and from 55.2 to values indicating no effect, respectively. The inhibition toward pancreatic lipase (IC < 5 mg mL ), acetylcholinesterase (ranged 9.15-22.2%) and butyrylcholinesterase (ranged 20.6-48.6%) was increased with the presence of total flavonoids and hydroxycinnamic acids content (identifying mainly in DS from quinces). It is noteworthy that none of the samples presented off-flavour notes, supporting the high quality of the products.
DS can be an innovative supplement to a diet as a snack used in the prevention of neurological changes and disorders of carbohydrate and lipid metabolism. © 2020 Society of Chemical Industry.
肥胖和 2 型糖尿病是目前最常见的慢性疾病，也是阿尔茨海默病，这是一种进行性神经退行性疾病。因此，寻找负责这些疾病发展的有效酶抑制剂是至关重要的。因此，本研究的目的是研究水果泥（榅桲、枣和苹果）和石榴汁品种（“Mollar de Elche”和“ Wonderful”）对石榴干片（DS）抑制与代谢相关的酶（α-淀粉酶、α-葡萄糖苷酶和胰脂肪酶活性）和神经紊乱（乙酰胆碱酯酶和丁酰胆碱酯酶活性）的影响。还研究了 DS 的质量特性（颜色坐标、质地特性和感官特性）。此外，还研究了储存条件（4 个月，4 和 20°C）对酚类含量的影响。
枣的 DS 具有最高的抗氧化能力，并且在酚类化合物方面表现出最高的储存稳定性。DS 的 α-淀粉酶和 α-葡萄糖苷酶半最大抑制浓度（IC50，mg/mL）抑制范围分别为 107-216 和 55.2-无影响值。对胰脂肪酶（IC50<5mg/mL）、乙酰胆碱酯酶（9.15-22.2%）和丁酰胆碱酯酶（20.6-48.6%）的抑制作用随着总黄酮和羟基肉桂酸含量的增加而增加（主要存在于榅桲的 DS 中）。值得注意的是，没有一个样品有异味，这支持了产品的高质量。
DS 可以作为一种创新的补充剂，作为零食用于预防神经变化和碳水化合物及脂质代谢紊乱。© 2020 化学工业协会。
