School of Chemistry and Molecular Engineering, Shanghai Key Laboratory for Urban Ecological Processes and Eco-Restoration, Engineering Research Center for Nanophotonics and Advanced Instrument, Ministry of Education, East China Normal University, 500 Dongchuan Road, Shanghai 200241, China.
Analyst. 2020 Nov 9;145(22):7191-7196. doi: 10.1039/d0an01641a.
In this work, we prepared a type of multiplexing upconversion nanoparticle (UCNP). There are three fluorescence emission peaks when our UCNPs are excited with 980 and 808 nm lasers. These fluorescence peaks of UCNPs can be quenched ("turn off") to varying degrees via fluorescence resonance energy transfer (FRET) when the UCNPs are coated with a polydopamine (PDA) layer, which is a universal quencher self-polymerized from dopamine (DA). Here, we create a novel single-component nanoprobe that can be used for the pattern recognition of antioxidants in a "turn on" manner by integrating with the prevention of PDA formation with an antioxidant. Our sensing strategy is based on the recovery of the fluorescence intensity of three emission peaks to different degrees due to different antioxidants with differential inhibition of PDA formation. Then, these three fluorescence emission peaks of UCNPs are innovatively selected as the sensor array, which enables us to discriminate multiple antioxidants and their mixtures. Simultaneously, the sensor array shows excellent performance in the chiral discrimination of cysteine enantiomers. This is a novel, innovative sensor array that requires only a single component to achieve the upconversion fluorescence pattern and recognize chiral molecules, and it elucidates a more innovative concept towards widespread applications.
在这项工作中，我们制备了一种多重上转换纳米粒子（UCNP）。当我们的 UCNP 被 980nm 和 808nm 激光激发时，会有三个荧光发射峰。当 UCNP 被聚多巴胺（PDA）层包裹时，这些 UCNP 的荧光峰可以通过荧光共振能量转移（FRET）被不同程度地猝灭（“关闭”），聚多巴胺是由多巴胺（DA）自聚合形成的通用猝灭剂。在这里，我们通过将抗氧化剂与 PDA 形成的抑制作用整合在一起，创建了一种新型的单组分纳米探针，它可以通过“开启”方式对抗氧化剂进行图案识别。我们的传感策略基于由于不同的抗氧化剂对 PDA 形成的抑制作用不同，三个发射峰的荧光强度会以不同的程度恢复。然后，我们创新性地选择了 UCNP 的这三个荧光发射峰作为传感器阵列，使其能够区分多种抗氧化剂及其混合物。同时，该传感器阵列在半胱氨酸对映体的手性识别方面表现出优异的性能。这是一种新颖的、创新的传感器阵列，仅需单一组件即可实现上转换荧光模式并识别手性分子，为更广泛的应用阐明了一个更具创新性的概念。
