Department of Animal Reproduction, Lasbela University of Agriculture, Water and Marine Sciences, Uthal, Pakistan.
Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, UPM, Serdang, Selangor, Malaysia.
Microb Pathog. 2020 Dec;149:104539. doi: 10.1016/j.micpath.2020.104539. Epub 2020 Sep 29.
Caseous lymphadenitis (CLA) caused by Corynebacterium pseudotuberculosis is characterized by the development of abscesses, mainly in superficial and internal lymph nodes, visceral and reproductive organs in small ruminants. This study aims to examine the histopathological changes in reproductive organs of goats immunized with killed vaccine of C. pseudotuberculosis. In this study, twenty four (24) clinically healthy bucks and does were divided into four groups A, B, C and D. Animals in groups A and B were immunized with 0.5 and 1% formalin killed vaccine, respectively; followed by a booster dose. After the booster dose of immunization, groups A, B and C were challenged with C. pseudotuberculosis at 10 cfu/ml. Goats in group D were immunize and unchallenged and left as control group. All C. pseudotuberculosis infected animals were euthanized humanely 12 weeks post-challenged. Tissue samples such as testes, epididymis, spermatic cord, penis, pituitary gland, mammary gland, vulva, vagina, cervix, uterus, fallopian tube and ovaries were collected for histopathology study. Microscopic examination of all tissues (testes, seminiferous tubules, spermatic cord, penile tissues and the pituitary gland) in the male reproductive organs of the bucks that were inoculated with 2 ml of 0.5% and 1.0% of C. pseudotuberculosis killed vaccine showed normal (animals inoculated with 1.0%) to mild (animals inoculated with 0.5%) histopathological changes when compared with those from group C which showed varying degrees of histopathological changes (p < 0.01) in their various tissues. For the female does, similar histopathological changes were observed for the various tissues examined (ovaries, fallopian tubes, uterine horns, uterine tissues, cervix, vaginal, vulva, mammary glands and the pituitary glands) in which the vaccinated groups A &B showed a significantly (p < 0.001) less histopathological changes when compared with those in group C that showed varying degrees of histopathological changes in the reproductive organs investigated. This study showed the efficacy of C. pseudotuberculosis killed vaccine protecting against reproductive tissue damages cause by the active infection with the live bacteria in both bucks and does in the study area.
干酪样淋巴结炎（CLA）由假结核棒状杆菌引起，其特征是在小反刍动物的浅表和内部淋巴结、内脏和生殖器官中形成脓肿。本研究旨在检查用假结核棒状杆菌死疫苗免疫的山羊生殖器官的组织病理学变化。在这项研究中，24 只（24）临床健康的公山羊和母山羊分为四组 A、B、C 和 D。A 组和 B 组的动物分别用 0.5%和 1%福尔马林灭活疫苗免疫；然后进行加强免疫。加强免疫后，A、B 和 C 组用 10cfu/ml 的假结核棒状杆菌进行攻毒。D 组的山羊免疫但不攻毒，作为对照组。所有感染假结核棒状杆菌的动物在攻毒后 12 周均人道处死。收集睾丸、附睪、精索、阴茎、脑垂体、乳腺、外阴、阴道、子宫颈、子宫、输卵管和卵巢等组织样本进行组织病理学研究。对注射 2ml 0.5%和 1.0%假结核棒状杆菌灭活疫苗的公山羊的雄性生殖器官的所有组织（睾丸、精曲小管、精索、阴茎组织和脑垂体）进行显微镜检查，与 C 组相比，结果显示正常（注射 1.0%的动物）到轻度（注射 0.5%的动物）组织病理学变化（p<0.01），在其各种组织中显示出不同程度的组织病理学变化。对于母山羊，在研究的各种组织中观察到类似的组织病理学变化（卵巢、输卵管、子宫角、子宫组织、子宫颈、阴道、外阴、乳腺和脑垂体），其中 A 组和 B 组的接种组与 C 组相比，组织病理学变化明显减少（p<0.001），C 组在研究的生殖器官中显示出不同程度的组织病理学变化。本研究表明，在研究区域内，假结核棒状杆菌灭活疫苗对活细菌引起的雄性和雌性生殖组织损伤具有保护作用。
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