Seyffert Nubia, Silva Renata Faria, Jardin Julien, Silva Wanderson Marques, Castro Thiago Luiz de Paula, Tartaglia Natayme Rocha, Santana Karina Talita de Oliveira, Portela Ricardo Wagner, Silva Artur, Miyoshi Anderson, Le Loir Yves, Azevedo Vasco
Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil; Institut National de la Recherche Agronomique, UMR1253, Science et Technologie du Lait et de l'Œuf, F-35042 Rennes, France; AGROCAMPUS OUEST, UMR1253, Science et Technologie du Lait et de l'Œuf, F-35042 Rennes, France.
Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
Vet Microbiol. 2014 Nov 7;174(1-2):255-60. doi: 10.1016/j.vetmic.2014.08.024. Epub 2014 Sep 6.
Caseous lymphadenitis (CLA) is a highly prevalent disease in goats and sheep worldwide, which is caused by Corynebacterium pseudotuberculosis. Although several prophylactic methods against CLA have been proposed previously, the identification of new C. pseudotuberculosis proteins that are really produced during the infectious process is still needed to improve efficiency and accuracy in vaccines and diagnostics. In this study, we used optimized conditions for serological proteome analysis (SERPA) in order to identify new immune-reactive proteins in C. pseudotuberculosis culture supernatants of two strains, 1002 and C231, isolated from goats and sheep, respectively. Using a sheep and goat serum pool, 13 novel immune-reactive exoproteins common to the two strains were identified. Four of these proteins present known functions and were already described as immune-reactive proteins in other microorganisms, whereas the other nine are of unknown function and show low similarity with proteins from other bacterial species. These data reveal promising targets for immunoprophylactic methods against CLA.
干酪性淋巴结炎（CLA）是一种在全球范围内的山羊和绵羊中高度流行的疾病，由伪结核棒状杆菌引起。尽管此前已经提出了几种针对CLA的预防方法，但仍需要鉴定在感染过程中真正产生的新的伪结核棒状杆菌蛋白，以提高疫苗和诊断的效率和准确性。在本研究中，我们使用了优化的血清蛋白质组分析（SERPA）条件，以鉴定分别从山羊和绵羊中分离出的1002和C231这两个菌株的伪结核棒状杆菌培养上清液中的新免疫反应蛋白。使用绵羊和山羊混合血清，鉴定出了这两个菌株共有的13种新型免疫反应性外蛋白。其中四种蛋白具有已知功能，并且在其他微生物中已被描述为免疫反应蛋白，而另外九种蛋白功能未知，并且与其他细菌物种的蛋白相似度较低。这些数据揭示了针对CLA的免疫预防方法的有前景的靶点。
