Shibata Y, Flanagan J E, Smith M M, Robinson F W, Kono T
Biochim Biophys Acta. 1987 Aug 7;902(1):154-8. doi: 10.1016/0005-2736(87)90147-7.
The sedimentation characteristics of vesicles associated with the insulin-sensitive intracellular glucose transporter from rat adipocytes were studied. The method used was sucrose density gradient centrifugation, which was carried out under non-equilibrium and equilibrium (isopycnic) conditions. The glucose transport activity was determined by the reconstitution method. As reported previously, the sedimentation velocity of the intracellular glucose-transport activity was considerably slower than that of the counterpart in the plasma membrane. It was found, however, that the specific gravity of the slow-sedimenting glucose-transport activity was almost identical to that of the activity in the plasma membrane (d = 1.118-1.122). It is concluded that the intracellular glucose transport activity is associated not with low-density microsomal vesicles, but with unidentified slow-sedimenting vesicles that have a specific gravity similar to that of the plasma membrane.
对与大鼠脂肪细胞中胰岛素敏感型细胞内葡萄糖转运体相关的囊泡的沉降特性进行了研究。所采用的方法是蔗糖密度梯度离心，该操作在非平衡和平衡（等密度）条件下进行。葡萄糖转运活性通过重组法测定。如先前报道，细胞内葡萄糖转运活性的沉降速度明显慢于质膜中相应活性的沉降速度。然而，发现沉降缓慢的葡萄糖转运活性的比重与质膜中活性的比重几乎相同（d = 1.118 - 1.122）。得出的结论是，细胞内葡萄糖转运活性并非与低密度微粒体囊泡相关，而是与比重与质膜相似的未鉴定的沉降缓慢的囊泡相关。
