Buchholtz F, Schneider F W
Biophys Chem. 1987 May 9;26(2-3):171-9. doi: 10.1016/0301-4622(87)80020-0.
A minimal mechanism is proposed which describes the transcriptional and translational processes for four phage proteins (RNA polymerase, DNase, primase and DNA polymerase) involved in T3/T7 DNA replication. Phage DNA replication is also included. It is shown how lag times may be incorporated into a kinetic mechanism. The distinct three-stage transport of phage DNA into the bacterial host (E. coli) is considered. DNA transport is assumed to be rate-determining for the transcription of class I and II proteins. Transcriptional and translational lag times have been calculated on the basis of available gene mapping of T7 phages. The kinetic behavior of T7 and T3 phage infection is practically identical. The hydrolysis of bacterial DNA by phage DNase (endonculease and exonuclease) as well as the subsequent phosphorylation to the deoxymononucleoside triphosphates are assumed to be rate-determining in phage DNA replication. Good agreement with experiment is obtained in our computer simulations.
提出了一种最小机制，该机制描述了参与T3/T7 DNA复制的四种噬菌体蛋白（RNA聚合酶、DNase、引发酶和DNA聚合酶）的转录和翻译过程。噬菌体DNA复制也包含在内。展示了如何将延迟时间纳入动力学机制。考虑了噬菌体DNA进入细菌宿主（大肠杆菌）的独特三阶段运输。假定DNA运输是I类和II类蛋白转录的速率决定因素。基于T7噬菌体可用的基因图谱计算了转录和翻译延迟时间。T7和T3噬菌体感染的动力学行为实际上是相同的。假定噬菌体DNase（内切核酸酶和外切核酸酶）对细菌DNA的水解以及随后向脱氧单磷酸三磷酸的磷酸化是噬菌体DNA复制的速率决定因素。在我们的计算机模拟中获得了与实验的良好一致性。
