Department for Otorhinolaryngology, LMU Klinikum, Marchioninistr, Ludwig-Maximilians-Universität München, 81377, Munich, Germany.
Department of Otorhinolaryngology, Georg August University, University Hospital Göttingen, Robert-Koch-Str. 40, 37075, Göttingen, Germany.
Eur Arch Otorhinolaryngol. 2021 Jul;278(7):2517-2528. doi: 10.1007/s00405-020-06389-7. Epub 2020 Oct 3.
Adenocarcinoma of the salivary glands is of low incidence and a broad range of histopathological subtypes. Cancer stem cell markers (CSC) might serve as novel prognostic parameters. To date, only a few studies examined the expression of CSC in adenocarcinoma of the salivary glands with diverging results. To further investigate the reliability in terms of prognostic value, a histopathological analysis of CSCs on a cohort of patients with adenocarcinomas of the major salivary glands was performed.
Tumor samples of 40 consecutive patients with adenocarcinoma of the major salivary gland treated with curative intend at one tertiary center were stained with the CSCs ALDH1, BMI-1, CD44, Nanog, and SOX2. Expression of these markers was correlated with clinicopathological parameters and survival estimates.
Correlation of high expression of ALDH1 with higher grading (p < 0.001) and high expression of CD44 with the localization of the neoplasm (p = 0.05), larger tumor size (p = 0.006), positive pN-category (p = 0.023), and advanced UICC stage (p = 0.002) was found. Furthermore, high expression of SOX2 correlated with a negative perineural invasion (p = 0.02). No significant correlation of any investigated marker with survival estimates was observed.
In conclusion, our study did not find a significant correlation of the investigated CSCs with survival estimates in adenocarcinoma of the major salivary glands. Recapitulating the results of our study in conjunction with data in the literature, the CSCs ALDH1, BMI-1, CD44, Nanog, and SOX2 do not seem to serve as reliable prognostic parameters in the treatment of adenocarcinoma of the salivary glands.
唾液腺癌的发病率较低，且具有广泛的组织病理学亚型。癌症干细胞标志物（CSC）可能成为新的预后参数。迄今为止，只有少数研究检查了唾液腺癌中 CSC 的表达，结果存在差异。为了进一步研究在预后价值方面的可靠性，对一组主要唾液腺癌腺癌患者的 CSC 进行了组织病理学分析。
对在一家三级中心接受根治性治疗的 40 例连续的主要唾液腺癌腺癌患者的肿瘤样本进行了 CSCs ALDH1、BMI-1、CD44、Nanog 和 SOX2 的染色。这些标志物的表达与临床病理参数和生存估计相关。
ALDH1 的高表达与较高的分级相关（p<0.001），CD44 的高表达与肿瘤的定位相关（p=0.05），肿瘤较大（p=0.006），阳性 pN 分类（p=0.023），和较晚期的 UICC 分期（p=0.002）。此外，SOX2 的高表达与无神经周围侵犯相关（p=0.02）。任何被调查的标志物与生存估计均无显著相关性。
总之，我们的研究没有发现研究中的 CSCs 与主要唾液腺癌的生存估计有显著相关性。结合我们的研究结果和文献中的数据，CSCs ALDH1、BMI-1、CD44、Nanog 和 SOX2 似乎不能作为唾液腺癌治疗的可靠预后参数。
