Andrews Research & Education Foundation, Gulf Breeze, Florida, U.S.A.
Arthrex, Naples, Florida, U.S.A.
Arthroscopy. 2021 Mar;37(3):893-900. doi: 10.1016/j.arthro.2020.09.035. Epub 2020 Oct 1.
To assess whether point-of-care devices designed for collecting cellular components from blood or bone marrow could be used to isolate viable stem cells from synovial fluid.
Male and female patients older than 18 years old with either an acute, anterior cruciate ligament (ACL) injury or knee osteoarthritis (OA) with a minimum estimated 20 mL of knee effusion volunteered. Ten patients with an ACL injury and 10 patients with OA were enrolled. Two milliliters of collected synovial effusion were analyzed and cultured for cellular content. The remaining fluid was combined with whole blood and processed using a buffy-coat based platelet-rich plasma (PRP) processing system. Specimens were analyzed for cell counts, colony-forming unit (CFU) assays, differentiation assays, and flow cytometry.
ACL effusion fluid contained 42.1 ± 20.7 CFU/mL and OA effusion fluid contained 65.4 ± 42.1 CFU/mL. After PRP processing, the counts in ACL-PRP were 101.6 ± 66.1 CFU/mL and 114.8 ± 73.4 CFU/mL in the OA-PRP. Cells showed tri-lineage differentiation potential when cultured under appropriate parameters. When analyzed with flow cytometry, >95% of cells produced with culturing expressed cell surface markers typically expressed by known stem cell populations, specifically CD45-, CD73+, CD29+, CD44+, CD105+, and CD90+.
Multipotent viable stem cells can be harvested from knee synovial fluid, associated with an ACL injury or OA, and concentrated with a buffy coat-based PRP-processing device.
PRP devices can be used to harvest stem cells from effusion fluids. Methods to use effusion fluid associated with an ACL injury and OA should be investigated further.
评估用于从血液或骨髓中采集细胞成分的即时检测设备是否可用于从滑液中分离有活力的干细胞。
年龄大于 18 岁的男性和女性患者，患有急性前交叉韧带（ACL）损伤或膝关节骨关节炎（OA），膝关节积液至少估计为 20 毫升，自愿参加。共纳入 10 例 ACL 损伤患者和 10 例 OA 患者。分析并培养收集的滑膜液的 2 毫升细胞含量。将剩余的液体与全血混合，并使用基于白细胞层的富血小板血浆（PRP）处理系统进行处理。对标本进行细胞计数、集落形成单位（CFU）测定、分化测定和流式细胞术分析。
ACL 积液中的 CFU 为 42.1±20.7/mL，OA 积液中的 CFU 为 65.4±42.1/mL。经过 PRP 处理后，ACL-PRP 中的计数为 101.6±66.1 CFU/mL，OA-PRP 中的计数为 114.8±73.4 CFU/mL。当在适当的参数下培养时，细胞显示出三系分化潜能。通过流式细胞术分析，在培养过程中表达细胞表面标志物的细胞>95%，这些标志物通常由已知的干细胞群表达，特别是 CD45-、CD73+、CD29+、CD44+、CD105+和 CD90+。
可以从与 ACL 损伤或 OA 相关的膝关节滑液中收获多能有活力的干细胞，并使用基于白细胞层的 PRP 处理设备进行浓缩。
PRP 设备可用于从积液中收获干细胞。应进一步研究与 ACL 损伤和 OA 相关的积液液方法。
