2017-2018 年俄罗斯下诺夫哥罗德地区流行的轮状病毒 A 株的基因型组合。

Genotype constellations of the rotavirus A strains circulating in Nizhny Novgorod, Russia, 2017-2018.

机构信息

Laboratory of Molecular Epidemiology of Viral Infections, I.N. Blokhina Nizhny Novgorod Research Institute of Epidemiology and Microbiology, Nizhny Novgorod, Russian Federation 603 950, 71 Malaya Yamskaya Str., Nizhny Novgorod, Russia.

出版信息

Infect Genet Evol. 2020 Nov;85:104578. doi: 10.1016/j.meegid.2020.104578. Epub 2020 Oct 1.

DOI:10.1016/j.meegid.2020.104578

PMID:33010418

Abstract

Currently, the full-genome-based classification is widely used to investigate rotavirus A (RVA) strains found in different countries around the world. However, the information on the full genotypes of rotaviruses circulating in Russia is limited. Using partial sequencing, this study determined the full genotype constellations of 15 RVA strains in total commonly detected in Nizhny Novgorod (European part of Russia) in 2017-2018, three from each of the following genotypes G1P[8], G4P[8], and G9P[8] and six from G2P[4]. There were two intergenogroup mono-reassortants possessing an identical genotype constellation of G4-P[8]-I1-R1-C1-M1-A1-N1-T1-E2-H1 with the DS-1-like NSP4 gene of probably local origin. A variety of subgenotype lineages and their combinations of Wa-like rotaviruses and genetic heterogeneity among G9P[8] and G1P[8] strains were shown on the basis of phylogenetic analysis of each gene. Moreover, two distinct co-circulating variants that differed in all 11 genome segments were found among DS-1-like rotaviruses.

摘要

目前，全基因组分类被广泛用于研究世界不同国家发现的轮状病毒 A（RVA）株。然而，关于在俄罗斯流行的轮状病毒的全基因型信息有限。本研究通过部分测序，确定了 2017-2018 年在俄罗斯下诺夫哥罗德（俄罗斯欧洲部分）总共检测到的 15 株 RVA 株的全基因型组合，其中每种基因型 G1P[8]、G4P[8]和 G9P[8]各有 3 株，G2P[4]有 6 株。有两个属间单重组体具有相同的 G4-P[8]-I1-R1-C1-M1-A1-N1-T1-E2-H1 基因型组合，其 DS-1 样 NSP4 基因可能具有本地起源。通过对每个基因的系统进化分析，显示了 Wa 样轮状病毒的各种亚基因型谱系及其组合以及 G9P[8]和 G1P[8]株之间的遗传异质性。此外，在 DS-1 样轮状病毒中发现了两种在所有 11 个基因组片段都不同的独特共同循环变体。