R&D Division, World Institute of Kimchi, Gwangju 61755, Republic of Korea.
R&D Division, World Institute of Kimchi, Gwangju 61755, Republic of Korea; Department of Food Science and Technology, Chonnam National University, Gwangju 61186, Republic of Korea.
Waste Manag. 2020 Dec;118:585-590. doi: 10.1016/j.wasman.2020.09.025. Epub 2020 Sep 30.
The present work describes the feasibility of coffee residue extracts as cryoprotective agents in the storage stability of freeze-dried lactic acid bacteria. Coffee residue extracts were extracted from coffee residue, produced after coffee extraction for coffee powder and instant coffee preparation, using an autoclave. Leuconostoc mesenteroides WiKim32 was selected to evaluate the ability of coffee residue extracts to protect bacteria during freeze-dried storage. The storage stability of freeze-dried Leu. mesenteroides WiKim32 with coffee residue extracts was comparable to those with commercial cryoprotective agents. Coffee residue extracts contributed to storage stability immediately after freeze-drying (61.2%) and subsequent storage (48.7%). Our data indicate that the protective effect of the coffee residue extracts is associated with ions, carbohydrates, and phenolic compounds. Coffee residue extracts are feasible materials, which can reduce the storage and distribution costs compared to commercial agents currently available.
本研究描述了咖啡渣提取物作为冷冻干燥乳酸菌贮藏稳定性中冷冻保护剂的可行性。采用高压釜从咖啡渣中提取咖啡渣提取物，咖啡渣是咖啡粉和速溶咖啡制备过程中提取咖啡后的产物。选用肠膜明串珠菌 WiKim32 来评估咖啡渣提取物在冷冻干燥储存过程中保护细菌的能力。含有咖啡渣提取物的冷冻干燥肠膜明串珠菌 WiKim32 的储存稳定性可与含有商业冷冻保护剂的相媲美。咖啡渣提取物在冷冻干燥后（61.2%）立即以及随后的储存（48.7%）过程中均有助于储存稳定性。我们的数据表明，咖啡渣提取物的保护作用与离子、碳水化合物和酚类化合物有关。与目前可用的商业制剂相比，咖啡渣提取物是一种可行的材料，可以降低储存和运输成本。
