Kim Ho Myeong, Jeong Seul-Gi, Hwang In Min, Park Hae Woong
Technology Innovation Research Division, World Institute of Kimchi, Gwangju 61755, Republic of Korea.
ACS Omega. 2021 Dec 15;6(51):35334-35341. doi: 10.1021/acsomega.1c04335. eCollection 2021 Dec 28.
Lactic acid bacteria produce various bioactive compounds widely used in human healthcare. However, studies on cryoprotective agents for the efficient storage of lactic acid bacteria after freeze-drying are still lacking. Here, we report the shelf-life extension effects of a highly efficient and eco-friendly cryoprotective agent and a cold adaptation method on WiKim31. Cold adaptation of WiKim31 increased exopolysaccharide expression in response to abiotic stress. As a possible cryoprotective agent, the citrus byproduct (CP) contains a variety of sugars, amino acids, and cations, exhibiting high antioxidant activity. WiKim31 powders formulated with CP or a mixture of soy powder (SP) and CP exhibited high cell viability at 58.3 and 76.3%, respectively, after 56 days of storage. These results indicate that CP can be efficiently used as a novel cryoprotective agent either alone or in combination with SP to improve the storage conditions of WiKim31 and preserve it longer.
乳酸菌会产生各种广泛应用于人类医疗保健的生物活性化合物。然而，关于冷冻干燥后高效储存乳酸菌的冷冻保护剂的研究仍然匮乏。在此，我们报告了一种高效且环保的冷冻保护剂和一种冷适应方法对WiKim31的保质期延长效果。WiKim31的冷适应增加了其对非生物胁迫的胞外多糖表达。作为一种可能的冷冻保护剂，柑橘副产品（CP）含有多种糖类、氨基酸和阳离子，具有高抗氧化活性。用CP或大豆粉（SP）与CP的混合物配制的WiKim31粉末在储存56天后分别表现出58.3%和76.3%的高细胞活力。这些结果表明，CP可以单独或与SP联合有效地用作新型冷冻保护剂，以改善WiKim31的储存条件并使其保存更长时间。
