Department of Agricultural Chemistry, National Taiwan University, No. 1, Sec. 4, Roosevelt Rd., Taipei 10617, Taiwan.
Department of Geography, National Taiwan University, No. 1, Sec. 4, Roosevelt Rd., Taipei 10617, Taiwan.
Environ Int. 2020 Dec;145:106128. doi: 10.1016/j.envint.2020.106128. Epub 2020 Oct 1.
Hexabromocyclododecane (HBCD) is one of the most frequently used brominated flame retardants (BFRs) in the industries nowadays. Despite being listed as persistent organic pollutant (POP), it is still in use until 2025. Because of its bio-accumulative and toxic characteristics, the applicable remediation approach is required. The aim of this study is to identify the microbial community from soil with HBCD degradation ability. The soil suspension and soil samples from Chiang Chun Soil and River Bank Soil showed to degrade HBCD by 60% 4 days after treatment, the debromination ratio was around 60%, and the total HBCD removal ratio reached 70% and 77.9%, respectively. The HBCD debromination metabolites, and oxidation metabolites were identified by GC-MS. The microbial taxonomic diversity was observed with DGGE approach to evaluate the effect of HBCD of microbial community. Bacillus spp. and Clostridium spp. were identified as the dominant microbes in the Chiang Chun Soil, but the amount of Bacillus spp. were showed to be affected by HBCD. In conclusion, HBCD could be removed by the microbial consortium in soil under aerobic culturing condition by various metabolic pathways.
六溴环十二烷（HBCD）是当今工业中最常用的溴系阻燃剂（BFRs）之一。尽管被列为持久性有机污染物（POP），但它仍在使用中，直到 2025 年。由于其具有生物累积性和毒性的特点，因此需要采用适用的修复方法。本研究旨在从具有 HBCD 降解能力的土壤中鉴定微生物群落。土壤悬液和来自 Chiang Chun 土壤和河岸土壤的土壤样品在处理 4 天后显示出 HBCD 的降解率为 60%，脱溴率约为 60%，总 HBCD 去除率分别达到 70%和 77.9%。通过 GC-MS 鉴定了 HBCD 的脱溴代谢物和氧化代谢物。通过 DGGE 方法观察微生物分类多样性，以评估 HBCD 对微生物群落的影响。芽孢杆菌属和梭菌属被鉴定为 Chiang Chun 土壤中的优势微生物，但 Bacillus spp. 的数量显示受到 HBCD 的影响。总之，在好氧培养条件下，土壤中的微生物群落可以通过多种代谢途径去除 HBCD。
