评估人腺病毒和非人类腺病毒载体制剂质量的一种潜在方法。
A potential approach for assessing the quality of human and nonhuman adenoviral vector preparations.
机构信息
Department of Comparative Pathobiology, Purdue Institute for Immunology, Inflammation and Infectious Disease and Purdue University Center for Cancer Research, College of Veterinary Medicine, Purdue University, West Lafayette, Indiana 47907, USA.
出版信息
Can J Vet Res. 2020 Oct;84(4):314-318.
Various types of human and nonhuman adenoviral (AdV) vectors are being used as gene delivery vectors in preclinical and clinical investigations. The objective of this study was to determine the ratio between the 2 best assays that would effectively address the variability in the titration of various AdV vectors in different cell lines and help obtain consistent results in preclinical and clinical studies using different AdV vectors. Here, we compared plaque-forming units, tissue culture infectious dose 50, focus-forming units (FFU), virus particle (VP) count, and genome copy number (GCN) of purified preparations of human AdV type C5, bovine AdV type 3, and porcine AdV type 3 to determine a correlation between infectious and noninfectious virus particles. Our results suggest that a VP:FFU or a VP:GCN ratio could accurately reflect the quality of an AdV preparation and could serve as an indicator to control batch-to-batch variability.
各种类型的人腺病毒（AdV）和非人类腺病毒（AdV）载体被用作临床前和临床研究中的基因传递载体。本研究的目的是确定两种最佳检测方法的比值，以有效地解决不同细胞系中不同 AdV 载体滴定的变异性问题，并帮助使用不同的 AdV 载体在临床前和临床研究中获得一致的结果。在这里，我们比较了人腺病毒 5 型、牛腺病毒 3 型和猪腺病毒 3 型的纯化制剂的噬斑形成单位、组织培养感染剂量 50、焦点形成单位（FFU）、病毒粒子（VP）计数和基因组拷贝数（GCN），以确定传染性和非传染性病毒粒子之间的相关性。我们的结果表明，VP:FFU 或 VP:GCN 比值可以准确反映 AdV 制剂的质量，并可作为控制批间变异性的指标。
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