Shiomitsu Keijiro, Bechtel Sandra M, Thompson Patrick M, Frasca Salvatore
Department of Veterinary Clinical Sciences, University of Florida, Gainsville, Florida 32610, USA.
Can J Vet Res. 2020 Oct;84(4):319-323.
Immunohistochemistry has been used extensively to evaluate protein expression in clinical and research settings. However, immunohistochemistry is not always successful in veterinary medicine due to the lack of reliable antibody options, poor tissue preservation, labor-intensive staining, and antigen-retrieval optimization processes. RNAscope hybridization (ISH) is a powerful technology that uses a specific sequence probe to identify targeted mRNA. In this study, we demonstrate RNAscope ISH in 4 common canine malignancies, which are traditionally diagnosed by histopathology and immunohistochemistry. Probes were designed for commonly targeted mRNA markers of neoplastic tumors; these included c-kit in mast cell tumor, microphthalmia-associated transcription factor in malignant melanoma, ionized calcium-binding adapter molecule-1 in histiocytic sarcoma, and alkaline phosphatase in osteosarcoma. A strong staining signal was obtained by these 4 targets in each canine malignancy. These results support the use of RNAscope ISH for definitive diagnosis in canine malignancies.
免疫组织化学已被广泛用于评估临床和研究环境中的蛋白质表达。然而，由于缺乏可靠的抗体选择、组织保存不佳、染色劳动强度大以及抗原修复优化过程，免疫组织化学在兽医学中并不总是成功的。RNAscope杂交（ISH）是一种强大的技术，它使用特定序列探针来识别靶向mRNA。在本研究中，我们展示了RNAscope ISH在4种常见犬类恶性肿瘤中的应用，这些肿瘤传统上通过组织病理学和免疫组织化学进行诊断。针对肿瘤性肿瘤常见的靶向mRNA标记设计了探针；这些包括肥大细胞瘤中的c-kit、恶性黑色素瘤中的小眼相关转录因子、组织细胞肉瘤中的离子钙结合衔接分子-1以及骨肉瘤中的碱性磷酸酶。在每种犬类恶性肿瘤中，这4个靶点均获得了强烈的染色信号。这些结果支持使用RNAscope ISH对犬类恶性肿瘤进行明确诊断。
