• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

长链非编码RNA LINC00261通过上调GATA6介导的DKK3来抑制前列腺癌的肿瘤发生。

Long noncoding RNA LINC00261 suppresses prostate cancer tumorigenesis through upregulation of GATA6-mediated DKK3.

作者信息

Li Yang, Li Hai, Wei Xin

机构信息

Department of Urology, China-Japan Union Hospital of Jilin University, No. 126, Xiantai Street, Changchun, 130033 Jilin People's Republic of China.

出版信息

Cancer Cell Int. 2020 Sep 30;20:474. doi: 10.1186/s12935-020-01484-5. eCollection 2020.

DOI:10.1186/s12935-020-01484-5
PMID:33013201
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7526381/
Abstract

BACKGROUND

Prostate cancer is one of the leading causes of cancer death in males. Recent studies have reported aberrant expression of lncRNAs in prostate cancer. This study explores the role of LINC00261 in prostate cancer progression.

METHODS

The differentially expressed genes, transcription factors, and lncRNAs related to prostate cancer were predicted by bioinformatics analysis. Prostate cancer tissue samples and cell lines were collected for the determination of the expression of LINC00261 by reverse transcription quantitative polymerase chain reaction. The binding capacity of LINC00261 to the transcription factor GATA6 was detected by RIP, and GATA6 binding to the DKK3 promoter region was assessed by ChIP. In addition, luciferase reporter system was used to verify whether LINC00261 was present at the DKK3 promoter. After gain- and loss-of function approaches, the effect of LINC00261 on prostate cancer in vitro and in vivo was assessed by the determination of cell proliferation, invasion and migration as well as angiogenesis.

RESULTS

LINC00261, GATA6, and DKK3 were poorly expressed in prostate cancer. LINC00261 could inhibit transcriptional expression of DKK3 by recruiting GATA6. Overexpression of LINC00261 inhibited prostate cancer cells proliferation, migration, and invasion as well as angiogenesis, which could be reversed by silencing DKK3. Furthermore, LINC00261 could also suppress the tumorigenicity of cancer cells in vivo.

CONCLUSIONS

Our study demonstrates the inhibitory role of LINC00261 in prostate cancer progression, providing a novel biomarker for early detection of prostate cancer.

摘要

背景

前列腺癌是男性癌症死亡的主要原因之一。最近的研究报道了lncRNAs在前列腺癌中的异常表达。本研究探讨了LINC00261在前列腺癌进展中的作用。

方法

通过生物信息学分析预测与前列腺癌相关的差异表达基因、转录因子和lncRNAs。收集前列腺癌组织样本和细胞系，采用逆转录定量聚合酶链反应测定LINC00261的表达。通过RIP检测LINC00261与转录因子GATA6的结合能力，通过ChIP评估GATA6与DKK3启动子区域的结合。此外，使用荧光素酶报告系统验证LINC00261是否存在于DKK3启动子处。在进行功能获得和功能缺失实验后，通过测定细胞增殖、侵袭、迁移以及血管生成来评估LINC00261对前列腺癌体内外的影响。

结果

LINC00261、GATA6和DKK3在前列腺癌中表达较低。LINC00261可通过招募GATA6抑制DKK3的转录表达。LINC00261的过表达抑制了前列腺癌细胞的增殖、迁移、侵袭以及血管生成，沉默DKK3可逆转这些作用。此外，LINC00261还可在体内抑制癌细胞的致瘤性。

结论

我们的研究证明了LINC00261在前列腺癌进展中的抑制作用，为前列腺癌的早期检测提供了一种新的生物标志物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3547/7526381/a10a6eba4204/12935_2020_1484_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3547/7526381/d57a58a737e7/12935_2020_1484_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3547/7526381/dd3a3d4e5ba3/12935_2020_1484_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3547/7526381/878b84de2ae4/12935_2020_1484_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3547/7526381/e778be70d382/12935_2020_1484_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3547/7526381/4490d5588c4d/12935_2020_1484_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3547/7526381/84301afaa324/12935_2020_1484_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3547/7526381/a10a6eba4204/12935_2020_1484_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3547/7526381/d57a58a737e7/12935_2020_1484_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3547/7526381/dd3a3d4e5ba3/12935_2020_1484_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3547/7526381/878b84de2ae4/12935_2020_1484_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3547/7526381/e778be70d382/12935_2020_1484_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3547/7526381/4490d5588c4d/12935_2020_1484_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3547/7526381/84301afaa324/12935_2020_1484_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3547/7526381/a10a6eba4204/12935_2020_1484_Fig7_HTML.jpg

相似文献

1
Long noncoding RNA LINC00261 suppresses prostate cancer tumorigenesis through upregulation of GATA6-mediated DKK3.长链非编码RNA LINC00261通过上调GATA6介导的DKK3来抑制前列腺癌的肿瘤发生。
Cancer Cell Int. 2020 Sep 30;20:474. doi: 10.1186/s12935-020-01484-5. eCollection 2020.
2
Long noncoding RNA LINC00261 upregulates ITIH5 to impair tumorigenic ability of pancreatic cancer stem cells.长链非编码RNA LINC00261上调ITIH5以损害胰腺癌干细胞的致瘤能力。
Cell Death Discov. 2021 Aug 26;7(1):220. doi: 10.1038/s41420-021-00575-0.
3
Decreased expression of the long noncoding RNA LINC00261 indicate poor prognosis in gastric cancer and suppress gastric cancer metastasis by affecting the epithelial-mesenchymal transition.长链非编码RNA LINC00261表达降低表明胃癌预后不良，并通过影响上皮-间质转化抑制胃癌转移。
J Hematol Oncol. 2016 Jul 21;9(1):57. doi: 10.1186/s13045-016-0288-8.
4
LINC00261 elevation inhibits angiogenesis and cell cycle progression of pancreatic cancer cells by upregulating SCP2 via targeting FOXP3.LINC00261 通过靶向 FOXP3 上调 SCP2 抑制胰腺癌细胞的血管生成和细胞周期进程。
J Cell Mol Med. 2021 Oct;25(20):9826-9836. doi: 10.1111/jcmm.16930. Epub 2021 Sep 19.
5
Linc00261 suppresses growth and metastasis of non-small cell lung cancer via repressing epithelial-mesenchymal transition.Linc00261 通过抑制上皮-间充质转化抑制非小细胞肺癌的生长和转移。
Eur Rev Med Pharmacol Sci. 2019 May;23(9):3829-3837. doi: 10.26355/eurrev_201905_17810.
6
LINC00261 regulates EBF1 to suppress malignant progression of thyroid cancer.LINC00261 通过调控 EBF1 抑制甲状腺癌的恶性进展。
Eur Rev Med Pharmacol Sci. 2021 Dec;25(24):7626-7634. doi: 10.26355/eurrev_202112_27609.
7
Linc00261 inhibits metastasis and the WNT signaling pathway of pancreatic cancer by regulating a miR‑552‑5p/FOXO3 axis.Linc00261通过调控miR-552-5p/FOXO3轴抑制胰腺癌的转移和WNT信号通路。
Oncol Rep. 2020 Mar;43(3):930-942. doi: 10.3892/or.2020.7480. Epub 2020 Jan 27.
8
LINC00261 suppresses human colon cancer progression via sponging miR-324-3p and inactivating the Wnt/β-catenin pathway.LINC00261 通过海绵吸附 miR-324-3p 和失活 Wnt/β-catenin 通路来抑制人结肠癌细胞的进展。
J Cell Physiol. 2019 Dec;234(12):22648-22656. doi: 10.1002/jcp.28831. Epub 2019 Jun 10.
9
Upregulated long noncoding RNA Linc00261 in pre-eclampsia and its effect on trophoblast invasion and migration via regulating miR-558/TIMP4 signaling pathway.上调的长非编码 RNA Linc00261 在子痫前期中的作用及其通过调节 miR-558/TIMP4 信号通路对滋养细胞侵袭和迁移的影响。
J Cell Biochem. 2019 Aug;120(8):13243-13253. doi: 10.1002/jcb.28598. Epub 2019 Mar 19.
10
The evolutionarily conserved long non-coding RNA LINC00261 drives neuroendocrine prostate cancer proliferation and metastasis via distinct nuclear and cytoplasmic mechanisms.进化上保守的长非编码 RNA LINC00261 通过不同的核和细胞质机制驱动神经内分泌前列腺癌的增殖和转移。
Mol Oncol. 2021 Jul;15(7):1921-1941. doi: 10.1002/1878-0261.12954. Epub 2021 Apr 26.

引用本文的文献

1
Potential role of DKK3 and WIF1 in prostate cancer: bioinformatics and clinical analysis.DKK3和WIF1在前列腺癌中的潜在作用：生物信息学与临床分析
Discov Oncol. 2025 Aug 27;16(1):1637. doi: 10.1007/s12672-025-03488-x.
2
Increasing linc00261 expression may predict well cancer prognosis, based on the meta-analysis.基于荟萃分析，增加linc00261的表达可能预示着良好的癌症预后。
Discov Oncol. 2025 Jul 1;16(1):1205. doi: 10.1007/s12672-025-02908-2.
3
GSFM: A genome-scale functional module transformation to represent drug efficacy for drug discovery.

本文引用的文献

1
Bone secreted factors induce cellular quiescence in prostate cancer cells.骨分泌因子诱导前列腺癌细胞的细胞静止。
Sci Rep. 2019 Dec 9;9(1):18635. doi: 10.1038/s41598-019-54566-4.
2
miR-1303 promotes the proliferation, migration and invasion of prostate cancer cells through regulating the Wnt/β-catenin pathway by targeting DKK3.微小RNA-1303通过靶向 Dickkopf 相关蛋白3调控Wnt/β-连环蛋白信号通路，促进前列腺癌细胞的增殖、迁移和侵袭。
Exp Ther Med. 2019 Dec;18(6):4747-4757. doi: 10.3892/etm.2019.8120. Epub 2019 Oct 23.
3
Upregulated long noncoding RNA Linc00261 in pre-eclampsia and its effect on trophoblast invasion and migration via regulating miR-558/TIMP4 signaling pathway.
GSFM：一种用于药物发现的、代表药物疗效的基因组规模功能模块转化。
Acta Pharm Sin B. 2025 Jan;15(1):133-150. doi: 10.1016/j.apsb.2024.08.017. Epub 2024 Aug 24.
4
Race-specific coregulatory and transcriptomic profiles associated with DNA methylation and androgen receptor in prostate cancer.与前列腺癌中 DNA 甲基化和雄激素受体相关的种族特异性共调控和转录组特征。
Genome Med. 2024 Apr 2;16(1):52. doi: 10.1186/s13073-024-01323-6.
5
GATA6 Suppresses Lung Adenocarcinoma Progression by Activating CFTR to Modulate Arachidonic Acid Metabolism.GATA6通过激活CFTR调节花生四烯酸代谢来抑制肺腺癌进展。
Comb Chem High Throughput Screen. 2025;28(4):582-591. doi: 10.2174/0113862073269158240122072743.
6
Niraparib restrains prostate cancer cell proliferation and metastasis and tumor growth in mice by regulating the lncRNA MEG3/miR-181-5p/GATA6 pathway.尼拉帕利通过调节长链非编码 RNA MEG3/miR-181-5p/GATA6 通路抑制前列腺癌细胞增殖、转移和肿瘤生长。
PeerJ. 2023 Nov 29;11:e16314. doi: 10.7717/peerj.16314. eCollection 2023.
7
NRF1 knockdown alleviates lipopolysaccharide-induced pulmonary inflammatory injury by upregulating DKK3 and inhibiting the GSK-3β/β-catenin pathway.NRF1 敲低通过上调 DKK3 并抑制 GSK-3β/β-catenin 通路缓解脂多糖诱导的肺炎症损伤。
Clin Exp Immunol. 2023 Dec 11;214(1):120-129. doi: 10.1093/cei/uxad071.
8
Importance of long non-coding RNAs in the pathogenesis, diagnosis, and treatment of prostate cancer.长链非编码RNA在前列腺癌发病机制、诊断及治疗中的重要性。
Front Oncol. 2023 Mar 21;13:1123101. doi: 10.3389/fonc.2023.1123101. eCollection 2023.
9
SGK2 promotes prostate cancer metastasis by inhibiting ferroptosis via upregulating GPX4.SGK2 通过上调 GPX4 促进前列腺癌转移来抑制铁死亡。
Cell Death Dis. 2023 Jan 31;14(1):74. doi: 10.1038/s41419-023-05614-5.
10
Murine Falcor/LL35 lncRNA Contributes to Glucose and Lipid Metabolism In Vitro and In Vivo.小鼠Falcor/LL35长链非编码RNA在体外和体内对葡萄糖及脂质代谢有作用。
Biomedicines. 2022 Jun 13;10(6):1397. doi: 10.3390/biomedicines10061397.
上调的长非编码 RNA Linc00261 在子痫前期中的作用及其通过调节 miR-558/TIMP4 信号通路对滋养细胞侵袭和迁移的影响。
J Cell Biochem. 2019 Aug;120(8):13243-13253. doi: 10.1002/jcb.28598. Epub 2019 Mar 19.
4
Deregulated GATA6 modulates stem cell-like properties and metabolic phenotype in hepatocellular carcinoma.GATA6 失调调节肝癌中的干细胞样特性和代谢表型。
Int J Cancer. 2019 Oct 1;145(7):1860-1873. doi: 10.1002/ijc.32248. Epub 2019 Mar 28.
5
Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries.全球癌症统计数据 2018：GLOBOCAN 对全球 185 个国家/地区 36 种癌症的发病率和死亡率的估计。
CA Cancer J Clin. 2018 Nov;68(6):394-424. doi: 10.3322/caac.21492. Epub 2018 Sep 12.
6
HOTAIR is a REST-regulated lncRNA that promotes neuroendocrine differentiation in castration resistant prostate cancer.HOTAIR 是一种 REST 调节的 lncRNA，可促进去势抵抗性前列腺癌的神经内分泌分化。
Cancer Lett. 2018 Oct 1;433:43-52. doi: 10.1016/j.canlet.2018.06.029. Epub 2018 Jun 23.
7
CRISPR-Mediated Reactivation of DKK3 Expression Attenuates TGF-β Signaling in Prostate Cancer.CRISPR介导的DKK3表达激活减弱前列腺癌中的TGF-β信号传导。
Cancers (Basel). 2018 May 28;10(6):165. doi: 10.3390/cancers10060165.
8
The Epidemiology of Prostate Cancer.前列腺癌的流行病学。
Cold Spring Harb Perspect Med. 2018 Dec 3;8(12):a030361. doi: 10.1101/cshperspect.a030361.
9
Dickkopf homolog 3 (DKK3): A candidate for detection and treatment of cancers?Dickkopf 同源物 3（DKK3）：癌症检测和治疗的候选物？
J Cell Physiol. 2018 Jun;233(6):4595-4605. doi: 10.1002/jcp.26313. Epub 2018 Jan 15.
10
Evaluation of fluorescence in situ hybridization techniques to study long non-coding RNA expression in cultured cells.评价荧光原位杂交技术在培养细胞中长链非编码 RNA 表达研究中的应用。
Nucleic Acids Res. 2018 Jan 9;46(1):e4. doi: 10.1093/nar/gkx946.