Vu Q A, Zhang D E, Chroneos Z C, Nelson D A
FEBS Lett. 1987 Aug 10;220(1):79-83. doi: 10.1016/0014-5793(87)80879-7.
n-Butyrate inhibits the histone deacetylase from higher cells, but has little effect on the enzyme activity in Saccharomyces cerevisiae. Spermine and spermidine were therefore tested as potential yeast deacetylase inhibitors and found to inhibit fully the enzyme at 2 and 5 mM, respectively. The utility of these inhibitors was demonstrated by showing that 2 mM spermine substantially increased the incorporation of [3H]acetate into histone in a yeast nuclear acetyltransferase assay.
正丁酸盐可抑制高等细胞中的组蛋白脱乙酰酶，但对酿酒酵母中的该酶活性影响甚微。因此，对精胺和亚精胺作为潜在的酵母脱乙酰酶抑制剂进行了测试，结果发现它们分别在2 mM和5 mM时能完全抑制该酶活性。在酵母核乙酰转移酶测定中，2 mM精胺能显著增加[3H]乙酸掺入组蛋白的量，这证明了这些抑制剂的效用。
