Lin Jie-Huan, Chen Fu-Nan, Wu Can-Xing, Hu Shu-Qiao, Ma Jun
Department of Cardiothoracic Surgery, The First Hospital of Longyan City, Longyan, Fujian 364000, P.R. China.
Oncol Lett. 2020 Dec;20(6):284. doi: 10.3892/ol.2020.12146. Epub 2020 Sep 23.
Long non-coding (lnc) RNAs serve crucial functions in human cancers. However, the involvement of the lncRNA B4GALT1-antisense RNA 1 (AS1) in non-small cell lung cancer (NSCLC) has not been extensively studied. Reverse transcription-quantitative PCR was performed to detect B4GALT1-AS1 levels in NSCLC tissues and cell lines. Potential influences of B4GALT1-AS1 on biological functions of NSCLC were assessed through a series of experiments, and the molecular mechanism was determined via RNA immunoprecipitation (RIP) and bioinformatics analyses. The results of the present study demonstrated that knockdown of B4GALT1-AS1 significantly attenuated the proliferative ability and clonality of H1299 and A549 cells. In the present study, B4GALT1-AS1 competed as an endogenous RNA by sequestering microRNA-30e (miR-30e) leading to an enhanced expression of SRY-box transcription factor 9 (SOX9). The effects of silencing B4GALT1-AS1 on NSCLC cells proliferation could be ameliorated by inhibiting miR-30e or restoring SOX9. Hence, B4GALT1-AS1 acted as a lncRNA that drives tumor progression in NSCLC via the regulation of the miR-30e/SOX9 axis. The findings of the present study indicated that the B4GALT1-AS1/miR-30e/SOX9 axis maybe an effective target for NSCLC treatment and management.
长链非编码（lnc）RNA在人类癌症中发挥着关键作用。然而，lncRNA B4GALT1反义RNA 1（AS1）在非小细胞肺癌（NSCLC）中的作用尚未得到广泛研究。通过逆转录定量PCR检测NSCLC组织和细胞系中B4GALT1-AS1的水平。通过一系列实验评估B4GALT1-AS1对NSCLC生物学功能的潜在影响，并通过RNA免疫沉淀（RIP）和生物信息学分析确定其分子机制。本研究结果表明，敲低B4GALT1-AS1可显著减弱H1299和A549细胞的增殖能力和克隆性。在本研究中，B4GALT1-AS1作为内源性RNA，通过隔离微小RNA-30e（miR-30e）发挥作用，导致SRY盒转录因子9（SOX9）表达增强。抑制miR-30e或恢复SOX9可改善沉默B4GALT1-AS1对NSCLC细胞增殖的影响。因此，B4GALT1-AS1作为一种lncRNA，通过调节miR-30e/SOX9轴驱动NSCLC的肿瘤进展。本研究结果表明，B4GALT1-AS1/miR-30e/SOX9轴可能是NSCLC治疗和管理的有效靶点。
