Wu Xiu-Ping, Xu Zhi-Qiang, Xie Wang-Mei, Lai Yao-Long, He Kai, Jiang Yan, Xu Zhen-Chao, Lin Yi-Na, Xie Yuan-Fu
Department of Breast Surgery, Zhengxing Hospital, Zhangzhou, Fujian 363000, P.R. China.
Department of Radiation Oncology, Zhangzhou Hospital Affiliated to Fujian Medical University, Zhangzhou, Fujian 363000, P.R. China.
Exp Ther Med. 2022 Jan;23(1):109. doi: 10.3892/etm.2021.11032. Epub 2021 Dec 2.
Long non-coding (lnc) RNAs play crucial functions in human cancer. However, until recently, the involvement of the lncRNA GAS6-AS1 in breast cancer (BCa) malignancy has not been studied exhaustively. The roles and underlying mode of action of GAS6-AS1 action in BCa progression were examined through functional experiments. A decline in GAS6-AS1 level led to a significant decrease in BCa cell proliferation, and the ability for colony formation. Here, GAS6-AS1 competed as endogenous RNA by sequestering microRNA-215-5p (miR-215-5p) causing an enhanced expression of SRY-box transcription factor 9 (SOX9). The effects of silencing GAS6-AS1 on BCa malignant phenotypes could be ameliorated by inhibiting miR-215-5p or restoring SOX9. Thus, GAS6-AS1 acted as a lncRNA that drives tumor in BCa, and enabled progression of BCa through miR-215-5p /SOX9 axis regulation. These outcomes show that the GAS6-AS1/miR-215-5p/SOX9 axis is a potentially effective target for cancer treatment and management.
长链非编码（lnc）RNA在人类癌症中发挥着关键作用。然而，直到最近，lncRNA GAS6-AS1在乳腺癌（BCa）恶性肿瘤中的作用尚未得到详尽研究。通过功能实验研究了GAS6-AS1在BCa进展中的作用及其潜在作用模式。GAS6-AS1水平的下降导致BCa细胞增殖及集落形成能力显著降低。在此，GAS6-AS1作为内源性RNA，通过隔离微小RNA-215-5p（miR-215-5p）发挥作用，导致SRY盒转录因子9（SOX9）表达增强。抑制miR-215-5p或恢复SOX9可改善沉默GAS6-AS1对BCa恶性表型的影响。因此，GAS6-AS1作为一种lncRNA在BCa中驱动肿瘤发生，并通过miR-215-5p/SOX9轴调控促进BCa进展。这些结果表明，GAS6-AS1/miR-215-5p/SOX9轴是癌症治疗和管理的一个潜在有效靶点。
