Xu Kaixiang, Zang Xiaohong, Peng Mian, Zhao Qian, Lin Binbin
Department of Quality Management, Yangtze River Pharmaceutical (Group) Co., Ltd., Taizhou, Jiangsu 225321, China.
Department of Pharmacy, Taizhou Hospital of Traditional Chinese Medicine, Taizhou, Jiangsu 225300, China.
Int J Hypertens. 2020 Sep 17;2020:6250425. doi: 10.1155/2020/6250425. eCollection 2020.
Magnesium lithospermate B (MLB) was shown to suppress oxidative stress and reduce hypertension, but the role of MLB in pregnancy-induced hypertension (PIH) remains unknown. The objective of this study was to demonstrate the effects of MLB on rats with PIH.
A total of 40 pregnant SD rats were selected, and 30 rats were orally given NG-nitro-L-arginine methyl ester (L-NAME, 60 mg/kg/day) to establish PIH rat models. Rats were equally divided into four groups: control, PIH, 5 mg/kg MLB, and 10 mg/kg MLB. MLB was consecutively administered into PIH rats for one week. The effects of MLB on mean arterial blood pressure (MAP), urine protein level, inflammation, and oxidative stress together with angiogenesis were analyzed.
MLB prevented the elevation in MAP and urine protein levels induced by L-NAME. The activities of inflammatory cytokines were highly increased in serum and placental tissues of PIH rats, while cotreatment with MLB partially reversed the activities of these cytokines. MLB also recovered the expression of reactive oxygen species (ROS) in plasma of PIH rats together with levels of oxidative stress and antioxidant capacity in the placenta of PIH rats. The decreased expressions of vascular endothelial growth factor (VEGF), endothelial nitric oxide synthase (eNOS), and NO observed in PIH rats were increased by MLB. In addition, 10 mg/kg MLB exhibited higher protective effects as compared to lower doses of 5 mg/kg.
This study demonstrated that pretreatment with MLB decreased MAP, inflammation, and oxidative stress in rats with gestational hypertension.
丹参素镁B（MLB）已被证明可抑制氧化应激并降低高血压，但MLB在妊娠高血压综合征（PIH）中的作用尚不清楚。本研究的目的是证明MLB对PIH大鼠的影响。
总共选择40只怀孕的SD大鼠，其中30只大鼠口服NG-硝基-L-精氨酸甲酯（L-NAME，60mg/kg/天）以建立PIH大鼠模型。大鼠被平均分为四组：对照组、PIH组、5mg/kg MLB组和10mg/kg MLB组。将MLB连续给予PIH大鼠一周。分析MLB对平均动脉血压（MAP）、尿蛋白水平、炎症、氧化应激以及血管生成的影响。
MLB可预防L-NAME诱导的MAP和尿蛋白水平升高。PIH大鼠血清和胎盘组织中炎性细胞因子的活性显著增加，而与MLB联合治疗可部分逆转这些细胞因子的活性。MLB还恢复了PIH大鼠血浆中活性氧（ROS）的表达以及PIH大鼠胎盘的氧化应激水平和抗氧化能力。MLB增加了PIH大鼠中观察到的血管内皮生长因子（VEGF）、内皮型一氧化氮合酶（eNOS）和NO的降低表达。此外，与5mg/kg的较低剂量相比，10mg/kg的MLB表现出更高的保护作用。
本研究表明，用MLB预处理可降低妊娠高血压大鼠的MAP、炎症和氧化应激。
