Sergio Cuellar-Rufino, Rossana Citlali Zepeda, Mónica Flores-Muñoz, Isela Santiago-Roque, Omar Arroyo-Helguera
Centro de Investigaciones Biomédicas, Universidad Veracruzana, Av. Luis Castelazo Ayala S/N, Col. Industrial Ánimas, Xalapa 91190, Veracruz, Mexico.
Instituto de Ciencias de la Salud, Universidad Veracruzana, Av. Luis Castelazo Ayala S/N, Col. Industrial Ánimas, Xalapa 91190, Veracruz, Mexico.
J Nutr Metab. 2020 Sep 16;2020:2302795. doi: 10.1155/2020/2302795. eCollection 2020.
Overweight and obesity are defined as excessive and abnormal fat accumulation that is harmful to health. This study analyzes the effect of different concentrations of the lugol solution (molecular iodine dissolved in potassium iodide) on lipolysis in cultured 3T3-L1-differentiated adipocytes. The mature adipocytes were treated with doses from 1 to 100 m of lugol for 0.5, 6, and 24 h. The results showed that mature adipocytes exposed to lugol decrease their viability and increase caspase-3 activity with a lethal dose (LD50) of 473 m. In mature adipocytes, lugol decreased the total intracellular lipid content, being significant at doses of 10 and 100 m after 6 and 24 h of treatment ( < 0.01), and the accumulation of intracellular triglycerides decreased after 24 h of exposure to lugol ( < 0.05). Lugol treatment significantly increases the release of glycerol to the culture medium ( < 0.05). The levels of adipocyte-specific transcription factors C/EBP- were downregulated and PPAR- upregulated after 30 min with lugol. These results indicate a lipolytic effect of lugol dependent on PPAR- and C/EBP- expression in mature 3T3-L1 adipocytes.
超重和肥胖被定义为对健康有害的过度和异常脂肪堆积。本研究分析了不同浓度的卢戈氏溶液（溶解在碘化钾中的分子碘）对培养的3T3-L1分化脂肪细胞脂解作用的影响。成熟脂肪细胞用1至100μM的卢戈氏溶液处理0.5、6和24小时。结果显示，暴露于卢戈氏溶液的成熟脂肪细胞其活力降低，半致死剂量（LD50）为473μM时半胱天冬酶-3活性增加。在成熟脂肪细胞中，卢戈氏溶液降低了细胞内总脂质含量，在处理6和24小时后，10和100μM剂量时具有显著性差异（P<0.01），并且暴露于卢戈氏溶液24小时后细胞内甘油三酯的积累减少（P<0.05）。卢戈氏溶液处理显著增加了培养基中甘油的释放（P<0.05）。用卢戈氏溶液处理30分钟后，脂肪细胞特异性转录因子C/EBP-α水平下调，PPAR-γ水平上调。这些结果表明卢戈氏溶液在成熟3T3-L1脂肪细胞中具有依赖于PPAR-γ和C/EBP-α表达的脂解作用。
