Russell P, Garland D, Zigler J S, Meakin S O, Tsui L C, Breitman M L
FASEB J. 1987 Jul;1(1):32-5. doi: 10.1096/fasebj.1.1.3301496.
Human lens gamma-crystallin obtained from the expression of a gene construct stably integrated into mouse L cells was incubated with ascorbate in the presence of iron and oxygen. The resulting oxidation of the gamma-crystallin led to more acidic species of this protein. These alterations were similar to the changes seen with aging in the human lens. The results suggest that oxidation of lens crystallins may be responsible for the changes seen on aging and cataract development and that ascorbate may contribute to these alterations.
将通过稳定整合到小鼠L细胞中的基因构建体表达获得的人晶状体γ-晶状体蛋白，在铁和氧存在的情况下与抗坏血酸一起孵育。γ-晶状体蛋白由此产生的氧化导致该蛋白质产生更多酸性种类。这些改变类似于人晶状体老化时出现的变化。结果表明，晶状体晶状体蛋白的氧化可能是老化和白内障发展过程中所见变化的原因，并且抗坏血酸可能促成了这些改变。
