Wenzhou Medical University First Affiliated Hospital, Wenzhou, Zhejiang Province 325000, China.
Wujiang District Hospital of Traditional Chinese Medicine, Suzhou City, Suzhou, Jiangsu Province 215000, China.
Biomed Res Int. 2020 Sep 18;2020:1507561. doi: 10.1155/2020/1507561. eCollection 2020.
This study was aimed at observing the effect Jiao-Tai-Wan in menopausal depression.
In this paper, we used ovariectomized mice subjected to chronic unpredictable stress as a menopausal depression model. After the chronic stress, mice were administrated with JTW (3.3 and 6.6mg/kg) and imipramine (10 mg/kg) for 14 days. On the 14th day, mice were subjected to the behavior test like the forced swim test, tail suspension test, and locomotor activity or were sacrificed to assess the protein changes in different brain regions.
The administration of JTW at doses of 3.3 and 6.6mg/kg (p.o.) significantly shortened the duration of immobility in forced swim and tail suspension tests. There was no obvious difference in locomotor activity among all the groups. The western blot analysis data indicated that treatment with JTW (3.3 and 6.6 mg/kg, p.o.) prominently increased the AR protein and the downstream protein ERK1/2 levels in the prefrontal cortex and hippocampus. However, the administration of JTW did not influence c-Fos protein in either the prefrontal cortex or hippocampus.
Our findings suggest that JTW plays a vital role in ameliorating menopausal depression symptoms in the AR-ERK1/2 pathway in the prefrontal cortex and hippocampus.
本研究旨在观察交泰丸对绝经后抑郁症的影响。
本文采用慢性不可预知应激致去卵巢小鼠建立绝经后抑郁症模型。慢性应激后，给予交泰丸（3.3 和 6.6mg/kg）和丙咪嗪（10mg/kg）灌胃治疗 14 天。第 14 天，进行强迫游泳试验、悬尾试验和自主活动等行为学测试，或处死小鼠以评估不同脑区的蛋白变化。
交泰丸（3.3 和 6.6mg/kg，po）给药可显著缩短强迫游泳和悬尾试验中小鼠的不动时间。各组的自主活动无明显差异。Western blot 分析数据表明，交泰丸（3.3 和 6.6mg/kg，po）治疗可显著增加前额叶皮质和海马中的 AR 蛋白和下游蛋白 ERK1/2 水平。然而，交泰丸给药并不影响前额叶皮质和海马中的 c-Fos 蛋白。
我们的研究结果表明，交泰丸在改善绝经后抑郁症症状中发挥了重要作用，其作用机制可能与 AR-ERK1/2 通路有关，涉及前额叶皮质和海马。
