Ghadiminejad I, Baum H
Hepatology. 1987 Jul-Aug;7(4):743-9. doi: 10.1002/hep.1840070421.
Studies with subfractions of Saccharomyces cerevisiae obtained by differential centrifugation showed only two primary biliary cirrhosis-specific antigens. These antigenic species were shown, using preabsorption studies, to have determinants cross-reactive with their mammalian counterpart. Distribution profiles of marker enzymes and primary biliary cirrhosis antigens between sucrose density gradient subcellular fractions of yeast showed that a relatively high concentration of primary biliary cirrhosis-specific antigens was associated with fractions containing plasma membranes, as well as those containing mitochondria. The possible cell-surface localization of the primary biliary cirrhosis antigens was further investigated using an indirect immunofluorescent technique on a number of different mammalian cells. Rat hepatoma cells, isolated rat hepatocytes and human polymorphonuclear leukocytes and lymphocytes stained positively with primary biliary cirrhosis sera, but not with normal sera or primary biliary cirrhosis sera preabsorbed with beef heart mitochondria. However, blood cells from primary biliary cirrhotic patients gave positive immunofluorescence in all tests, which is compatible with prior binding of the patients' own antimitochondrial antibodies to the surface of the cells.
通过差速离心获得的酿酒酵母亚组分研究仅显示出两种原发性胆汁性肝硬化特异性抗原。利用吸收前研究表明，这些抗原种类具有与其哺乳动物对应物交叉反应的决定簇。酵母蔗糖密度梯度亚细胞组分之间标记酶和原发性胆汁性肝硬化抗原的分布情况表明，相对高浓度的原发性胆汁性肝硬化特异性抗原与含有质膜以及含有线粒体的组分相关。使用间接免疫荧光技术在多种不同哺乳动物细胞上进一步研究了原发性胆汁性肝硬化抗原可能的细胞表面定位。大鼠肝癌细胞、分离的大鼠肝细胞以及人多形核白细胞和淋巴细胞用原发性胆汁性肝硬化血清染色呈阳性，但用正常血清或用牛心线粒体吸收的原发性胆汁性肝硬化血清染色则呈阴性。然而，原发性胆汁性肝硬化患者的血细胞在所有测试中均呈现阳性免疫荧光，这与患者自身抗线粒体抗体预先结合到细胞表面相符。
