Dmitriev B A, Knirel Y A, Kochetkov N K, Hofman I L, Capek K
Eur J Biochem. 1977 Jun 15;76(2):433-40. doi: 10.1111/j.1432-1033.1977.tb11612.x.
The O-specific polysaccharide obtained from Shigella dysenteriae type-2 lipopolysaccharide by mild acid hydrolysis consisted of N-acetylgalactosamine, N-acetylglucosamine, D-galactose, D-glucose, and O-acetyl group in the ratio of 2:1:1:1:1. A number of oligosaccharides were obtained by deamination of the N-deacetylated polysaccharide and by Smith degradation of the both native and O-deacetylated polysaccharides. The identification of oligosaccharides along with methylation analysis and chromic anhydride oxidation showed that the polysaccharide was built up of the repeating pentasaccharide units whose proposed structure is given below: (see article) Serological properties of Sh. dysenteriae O-specific polysaccharides are discussed.
通过温和酸水解从痢疾志贺氏菌2型脂多糖获得的O-特异性多糖由N-乙酰半乳糖胺、N-乙酰葡糖胺、D-半乳糖、D-葡萄糖和O-乙酰基按2:1:1:1:1的比例组成。通过N-脱乙酰化多糖的脱氨基作用以及天然和O-脱乙酰化多糖的史密斯降解获得了许多寡糖。寡糖的鉴定以及甲基化分析和铬酸酐氧化表明，该多糖由重复的五糖单元组成，其推测结构如下：（见文章）讨论了痢疾志贺氏菌O-特异性多糖的血清学性质。
