te Velde A A, Keizer G D, Figdor C G
Immunology. 1987 Jul;61(3):261-7.
Human peripheral blood monocytes from normal, healthy donors express the leucocyte function-associated antigen (LFA)-1, CR3 and p150,95. These heterodimeric antigens are members of a glycoprotein family sharing a common beta subunit but endowed with distinct alpha chains. They have been shown to play an important role in cell-cell interactions. In the present study we have investigated the role of these molecules in the interaction of monocytes with endothelial cells and melanoma (tumour) cells. Heterotypic cell-cell interactions were studied in single cell conjugate assays and by adhesion of monocytes to monolayers of cells. The results demonstrate that monoclonal antibodies directed against LFA-1 alpha, CR3 alpha, p150,95 alpha and the common beta chain strongly reduce the number of conjugates (71, 50, 60 and 89% inhibition, respectively), formed between monocytes and melanoma or endothelial cells in a single cell assay. In contrast, adhesion of monocytes to monolayers of the same cells seems only to depend on p150,95, since only antibodies directed to the alpha chain of this molecule and to the common beta chain inhibited adhesion. Interestingly, the number of conjugates formed with melanoma cells in single cell assays was at least twice the number of conjugates formed between monocytes and endothelial cells, whereas no differences were observed in the adhesion of monocytes to monolayers of these cells. However, the basis for this phenomenon is not yet clear. These results indicate that not only LFA-1 but also CR3 and p150,95 can mediate adhesion to target cells in suspension, but that monocyte adhesion to monolayers is caused by a different mechanism in which the p150,95 molecule seems to play a prominent role.
来自正常健康供体的人外周血单核细胞表达白细胞功能相关抗原（LFA）-1、CR3和p150,95。这些异二聚体抗原是糖蛋白家族的成员，它们共享一个共同的β亚基，但具有不同的α链。它们已被证明在细胞间相互作用中起重要作用。在本研究中，我们研究了这些分子在单核细胞与内皮细胞和黑色素瘤（肿瘤）细胞相互作用中的作用。在单细胞结合试验和单核细胞与单层细胞的黏附中研究了异型细胞间相互作用。结果表明，针对LFA-1α、CR3α、p150,95α和共同β链的单克隆抗体在单细胞试验中能显著减少单核细胞与黑色素瘤或内皮细胞之间形成的结合物数量（分别抑制71%、50%、60%和89%）。相比之下，单核细胞与相同细胞单层的黏附似乎仅取决于p150,95，因为只有针对该分子α链和共同β链的抗体能抑制黏附。有趣的是，在单细胞试验中与黑色素瘤细胞形成的结合物数量至少是单核细胞与内皮细胞之间形成的结合物数量的两倍，而在单核细胞与这些细胞单层的黏附中未观察到差异。然而，这种现象的基础尚不清楚。这些结果表明，不仅LFA-1，而且CR3和p150,95都可以介导对悬浮靶细胞的黏附，但单核细胞与单层的黏附是由一种不同的机制引起的，其中p150,95分子似乎起主要作用。
