Vähätupa Maria, Jääskeläinen Niina, Cerrada-Gimenez Marc, Thapa Rubina, Järvinen Tero, Kalesnykas Giedrius, Uusitalo-Järvinen Hannele
Faculty of Medicine and Health Technology, Tampere University & Tampere University Hospital; Experimentica Ltd.
Experimentica Ltd.
J Vis Exp. 2020 Sep 16(163). doi: 10.3791/61482.
One of the commonly used models for ischemic retinopathies is the oxygen-induced retinopathy (OIR) model. Here we describe detailed protocols for the OIR model induction and its readouts in both mice and rats. Retinal neovascularization is induced in OIR by exposing rodent pups either to hyperoxia (mice) or alternating levels of hyperoxia and hypoxia (rats). The primary readouts of these models are the size of neovascular (NV) and avascular (AVA) areas in the retina. This preclinical in vivo model can be used to evaluate the efficacy of potential anti-angiogenic drugs or to address the role of specific genes in the retinal angiogenesis by using genetically manipulated animals. The model has some strain and vendor specific variation in the OIR induction which should be taken into consideration when designing the experiments.
缺血性视网膜病变常用的模型之一是氧诱导性视网膜病变（OIR）模型。在此，我们描述了在小鼠和大鼠中诱导OIR模型及其读数的详细方案。通过将幼鼠暴露于高氧环境（小鼠）或交替的高氧和低氧环境（大鼠），在OIR中诱导视网膜新生血管形成。这些模型的主要读数是视网膜中新生血管（NV）和无血管（AVA）区域的大小。这种临床前体内模型可用于评估潜在抗血管生成药物的疗效，或通过使用基因操作动物来研究特定基因在视网膜血管生成中的作用。该模型在OIR诱导方面存在一些品系和供应商特异性差异，在设计实验时应予以考虑。
