Osuntokun Opeyemi S, Olayiwola Gbola, Atere Tope G, Adekomi Damilare A, Adedokun Kabiru I, Oladokun Olayemi O
Department of Physiology, Faculty of Basic Medical Sciences, College of Health Sciences, Osun State University, Osogbo, Nigeria.
Department of Clinical Pharmacy and Pharmacy Administration, Faculty of Pharmacy, Obafemi Awolowo University, Ile-Ife, Nigeria.
Andrologia. 2020 Dec;52(11):e13788. doi: 10.1111/and.13788. Epub 2020 Oct 5.
The effects of phenytoin (PHT), levetiracetam (LEV) or their adjunctive treatment on the hypothalamic-pituitary-testicular axis in male Wistar rats were determined. Twenty-eight male rats (150-180 g) were randomised into four groups (N = 7). Groups I to IV received intraperitoneal treatment of either normal saline (0.2 ml i.p) or PHT (50 mg/kg i.p) or LEV (50 mg/kg) or PHT (25 mg/kg) and LEV (25 mg/kg) combination for 28 days. The organ weight, concentrations of reproductive hormones and semen profile were determined, while the brain, epididymis and testis were preserved in 10% neutral-buffered formalin for the histomorphological study. Data were analysed using descriptive and inferential statistics. The results were presented as mean ± SEM in graphs or tables, while the level of significance was taken at p < .05. The semen profile was altered significantly in all the treatment groups. The gonadotrophic releasing hormone, luteinising hormone and testosterone concentration decreased significantly in the PHT- and PHT + LEV-treated groups compared with control. There were various disruptions in the hypothalamus, pituitary and testis of the PHT- and PHT + LEV adjunctive-treated rats. In conclusion, chronic PHT + LEV treatment may pose deleterious effects on the hypothalamic-pituitary-testicular axis than single treatment of either of the two drugs in male rats.
研究了苯妥英（PHT）、左乙拉西坦（LEV）或它们的联合治疗对雄性Wistar大鼠下丘脑-垂体-睾丸轴的影响。将28只雄性大鼠（150 - 180克）随机分为四组（每组n = 7）。第一组至第四组分别腹腔注射生理盐水（0.2毫升腹腔注射）或PHT（50毫克/千克腹腔注射）或LEV（50毫克/千克）或PHT（25毫克/千克）与LEV（25毫克/千克）的组合，持续28天。测定了器官重量、生殖激素浓度和精液参数，同时将大脑、附睾和睾丸保存在10%中性缓冲福尔马林中用于组织形态学研究。使用描述性和推断性统计分析数据。结果以图表中的平均值±标准误表示，显著性水平设定为p < 0.05。所有治疗组的精液参数均有显著改变。与对照组相比，PHT组和PHT + LEV组的促性腺激素释放激素、黄体生成素和睾酮浓度显著降低。PHT组和PHT + LEV联合治疗组的大鼠下丘脑、垂体和睾丸存在各种结构破坏。总之，与单独使用这两种药物之一相比，慢性PHT + LEV治疗对雄性大鼠下丘脑-垂体-睾丸轴可能产生有害影响。
