Sa Kyu Jin, Choi Ik-Young, Lee Ju Kyong
Department of Applied Plant Sciences, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon 24341, Korea.
Department of Agriculture and Life Industry, Kangwon National University, Chuncheon 24341, Korea.
Genomics Inform. 2020 Sep;18(3):e29. doi: 10.5808/GI.2020.18.3.e29. Epub 2020 Sep 9.
Maize seed pigmentation is one of the important issue to develop maize seed breeding. The differently gene expression was characterized and compared for three inbred lines, such as the pigment accumulated seed (CM22) and non-pigmented seed (CM5 and CM19) at 10 days after pollination. We obtained a total of 63,870, 82,496, and 54,555 contigs by de novo assembly to identify gene expression in the CM22, CM5, and CM19, respectably. In differentially expressed gene analysis, it was revealed that 7,044 genes were differentially expressed by at least two-fold, with 4,067 upregulated in colored maize inbred lines and 2,977 upregulated in colorless maize inbred lines. Of them,18 genes were included to the anthocyanin biosynthesis pathways, while 15 genes were upregulated in both CM22/5 and CM22/19. Additionally, 37 genes were detected in the metabolic pathway concern to the seed pigmentation by BINs analysis using MAPMAN software. Finally, these differently expressed genes may aid in the research on seed pigmentation in maize breeding programs.
玉米种子色素沉着是玉米种子育种发展中的重要问题之一。在授粉后10天，对三个自交系进行了不同基因表达的表征和比较，如色素积累种子（CM22）和非色素种子（CM5和CM19）。通过从头组装，我们分别获得了63,870、82,496和54,555个重叠群，以鉴定CM22、CM5和CM19中的基因表达。在差异表达基因分析中，发现至少有7,044个基因差异表达了至少两倍，其中4,067个在有色玉米自交系中上调，2,977个在无色玉米自交系中上调。其中，18个基因参与花青素生物合成途径，15个基因在CM22/5和CM22/19中均上调。此外，使用MAPMAN软件通过BINs分析在与种子色素沉着相关的代谢途径中检测到37个基因。最后，这些差异表达基因可能有助于玉米育种计划中种子色素沉着的研究。
