• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

大肠杆菌中染色体与微型染色体复制的协同起始

Coordinate initiation of chromosome and minichromosome replication in Escherichia coli.

作者信息

Helmstetter C E, Leonard A C

出版信息

J Bacteriol. 1987 Aug;169(8):3489-94. doi: 10.1128/jb.169.8.3489-3494.1987.

DOI:10.1128/jb.169.8.3489-3494.1987
PMID:3301802
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC212423/
Abstract

Escherichia coli minichromosomes harboring as little as 327 base pairs of DNA from the chromosomal origin of replication (oriC) were found to replicate in a discrete burst during the division cycle of cells growing with generation times between 25 and 60 min at 37 degrees C. The mean cell age at minichromosome replication coincided with the mean age at initiation of chromosome replication at all growth rates, and furthermore, the age distributions of the two events were indistinguishable. It is concluded that initiation of replication from oriC is controlled in the same manner on minichromosomes and chromosomes over the entire range of growth rates and that the timing mechanism acts within the minimal oriC nucleotide sequence required for replication.

摘要

发现在37℃下，世代时间在25至60分钟之间生长的细胞分裂周期中，携带来自染色体复制起点（oriC）低至327个碱基对DNA的大肠杆菌微型染色体以离散爆发的方式进行复制。在所有生长速率下，微型染色体复制时的平均细胞年龄与染色体复制起始时的平均年龄一致，此外，这两个事件的年龄分布无法区分。得出的结论是，在整个生长速率范围内，oriC的复制起始在微型染色体和染色体上以相同方式受到控制，并且时间调控机制在复制所需的最小oriC核苷酸序列内起作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e92f/212423/5b23fd367ed9/jbacter00198-0106-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e92f/212423/5b23fd367ed9/jbacter00198-0106-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e92f/212423/5b23fd367ed9/jbacter00198-0106-a.jpg

相似文献

1
Coordinate initiation of chromosome and minichromosome replication in Escherichia coli.大肠杆菌中染色体与微型染色体复制的协同起始
J Bacteriol. 1987 Aug;169(8):3489-94. doi: 10.1128/jb.169.8.3489-3494.1987.
2
Cell cycle-specific replication of Escherichia coli minichromosomes.大肠杆菌微型染色体的细胞周期特异性复制。
Proc Natl Acad Sci U S A. 1986 Jul;83(14):5101-5. doi: 10.1073/pnas.83.14.5101.
3
Different effects of mioC transcription on initiation of chromosomal and minichromosomal replication in Escherichia coli.mioC转录对大肠杆菌染色体和微型染色体复制起始的不同影响。
Nucleic Acids Res. 1992 Jun 25;20(12):3029-36. doi: 10.1093/nar/20.12.3029.
4
Maintenance and incompatibility of plasmids carrying the replication origin of the Escherichia coli chromosome: evidence for a control region of replication between oriC and asnA.携带大肠杆菌染色体复制起点的质粒的维持与不相容性：oriC和asnA之间存在复制控制区域的证据
Nucleic Acids Res. 1983 Aug 25;11(16):5775-91. doi: 10.1093/nar/11.16.5775.
5
Chromosomal replication incompatibility in Dam methyltransferase deficient Escherichia coli cells.Dam甲基转移酶缺陷型大肠杆菌细胞中的染色体复制不相容性。
EMBO J. 1996 Nov 1;15(21):5999-6008.
6
DnaA protein overproduction abolishes cell cycle specificity of DNA replication from oriC in Escherichia coli.DnaA蛋白过量表达消除了大肠杆菌中oriC处DNA复制的细胞周期特异性。
J Bacteriol. 1989 Jul;171(7):3760-6. doi: 10.1128/jb.171.7.3760-3766.1989.
7
Novel Escherichia coli mutant, dnaR, thermosensitive in initiation of chromosome replication.新型大肠杆菌突变体dnaR，在染色体复制起始过程中对温度敏感。
J Mol Biol. 1992 Aug 20;226(4):979-87. doi: 10.1016/0022-2836(92)91046-r.
8
Kinetics of minichromosome replication in Escherichia coli B/r.大肠杆菌B/r中微型染色体复制的动力学
J Bacteriol. 1982 Feb;149(2):499-507. doi: 10.1128/jb.149.2.499-507.1982.
9
Expression of accumulated capacity for initiation of chromosome and minichromosome replication in dnaA mutants of Escherichia coli.大肠杆菌dnaA突变体中染色体和微型染色体复制起始累积能力的表达
J Bacteriol. 1983 Jun;154(3):1371-80. doi: 10.1128/jb.154.3.1371-1380.1983.
10
Host controlled plasmid replication: Escherichia coli minichromosomes.宿主控制的质粒复制：大肠杆菌微型染色体
Plasmid. 2004 Nov;52(3):151-68. doi: 10.1016/j.plasmid.2004.08.001.

引用本文的文献

1
Bacterial Replication Initiation as Precision Control by Protein Counting.细菌复制起始的蛋白质计数精确调控
PRX Life. 2023 Aug-Oct;1(1). doi: 10.1103/prxlife.1.013011. Epub 2023 Aug 28.
2
Recollections of a Helmstetter Disciple.一位赫尔姆斯泰特弟子的回忆
Life (Basel). 2023 Apr 30;13(5):1114. doi: 10.3390/life13051114.
3
Blocking, Bending, and Binding: Regulation of Initiation of Chromosome Replication During the Cell Cycle by Transcriptional Modulators That Interact With Origin DNA.阻断、弯曲与结合：与起始点DNA相互作用的转录调节因子对细胞周期中染色体复制起始的调控

本文引用的文献

1
Kinetics of minichromosome replication in Escherichia coli B/r.大肠杆菌B/r中微型染色体复制的动力学
J Bacteriol. 1982 Feb;149(2):499-507. doi: 10.1128/jb.149.2.499-507.1982.
2
Growth rate-dependent control of chromosome replication initiation in Escherichia coli.大肠杆菌中染色体复制起始的生长速率依赖性控制
J Bacteriol. 1981 Mar;145(3):1232-8. doi: 10.1128/jb.145.3.1232-1238.1981.
3
Replication origin of the Escherichia coli K-12 chromosome: the size and structure of the minimum DNA segment carrying the information for autonomous replication.
Front Microbiol. 2021 Sep 20;12:732270. doi: 10.3389/fmicb.2021.732270. eCollection 2021.
4
Low Affinity DnaA-ATP Recognition Sites in Make Non-equivalent and Growth Rate-Dependent Contributions to the Regulated Timing of Chromosome Replication.基因组中低亲和力的DnaA-ATP识别位点对染色体复制的调控时间做出了不等同且依赖生长速率的贡献。
Front Microbiol. 2018 Jul 26;9:1673. doi: 10.3389/fmicb.2018.01673. eCollection 2018.
5
Fundamental principles in bacterial physiology-history, recent progress, and the future with focus on cell size control: a review.细菌生理学基础——历史、最新进展及未来展望，重点关注细胞大小控制：综述。
Rep Prog Phys. 2018 May;81(5):056601. doi: 10.1088/1361-6633/aaa628. Epub 2018 Jan 9.
6
Interrogating the Escherichia coli cell cycle by cell dimension perturbations.通过细胞尺寸扰动研究大肠杆菌细胞周期
Proc Natl Acad Sci U S A. 2016 Dec 27;113(52):15000-15005. doi: 10.1073/pnas.1617932114. Epub 2016 Dec 12.
7
(p)ppGpp and the bacterial cell cycle.（p）ppGpp与细菌细胞周期
J Biosci. 2016 Jun;41(2):277-82. doi: 10.1007/s12038-016-9611-3.
8
Initiation of DNA Replication.DNA复制的起始
EcoSal Plus. 2010 Sep;4(1). doi: 10.1128/ecosalplus.4.4.1.
9
The precarious prokaryotic chromosome.易变的原核染色体。
J Bacteriol. 2014 May;196(10):1793-806. doi: 10.1128/JB.00022-14. Epub 2014 Mar 14.
10
Regulating DNA replication in bacteria.调控细菌中的 DNA 复制。
Cold Spring Harb Perspect Biol. 2013 Apr 1;5(4):a012922. doi: 10.1101/cshperspect.a012922.
大肠杆菌K-12染色体的复制起点：携带自主复制信息的最小DNA片段的大小和结构
Mol Gen Genet. 1980 Apr;178(1):9-20. doi: 10.1007/BF00267207.
4
Determination of plasmid copy number by fluorescence densitometry.通过荧光密度测定法测定质粒拷贝数。
Plasmid. 1983 Mar;9(2):182-90. doi: 10.1016/0147-619x(83)90019-7.
5
Purified dnaA protein in initiation of replication at the Escherichia coli chromosomal origin of replication.纯化的DnaA蛋白在大肠杆菌染色体复制起点处启动复制过程中的作用。
Proc Natl Acad Sci U S A. 1983 Oct;80(19):5817-21. doi: 10.1073/pnas.80.19.5817.
6
Maintenance and incompatibility of plasmids carrying the replication origin of the Escherichia coli chromosome: evidence for a control region of replication between oriC and asnA.携带大肠杆菌染色体复制起点的质粒的维持与不相容性：oriC和asnA之间存在复制控制区域的证据
Nucleic Acids Res. 1983 Aug 25;11(16):5775-91. doi: 10.1093/nar/11.16.5775.
7
The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.pUC质粒，一种源自M13mp7的用于插入诱变和使用合成通用引物进行测序的系统。
Gene. 1982 Oct;19(3):259-68. doi: 10.1016/0378-1119(82)90015-4.
8
Regulation of plasmid replication.质粒复制的调控
Microbiol Rev. 1984 Mar;48(1):1-23. doi: 10.1128/mr.48.1.1-23.1984.
9
A system to study promoter and terminator signals recognized by Escherichia coli RNA polymerase.一种用于研究大肠杆菌RNA聚合酶识别的启动子和终止子信号的系统。
Gene Amplif Anal. 1981;2:383-415.
10
Control of DNA replication by membrane.膜对DNA复制的调控
Nature. 1968 Aug 3;219(5153):485-6. doi: 10.1038/219485a0.