Epps D E, Poorman R, Hui J, Carlson W, Heinrikson R
J Biol Chem. 1987 Aug 5;262(22):10570-3.
The fluorescence quenching of the three tryptophan residues of recombinant human renin was determined using ionic and penetrating quenchers. Tryptophans 44,200, and 312 of recombinant human renin were found to be totally inaccessible to the ionic quenchers cesium and iodide and only partially accessible to the penetrating quencher acrylamide. The renin had a fluorescence emission maximum at 325 nm which was made up of three separate components as determined by second derivative spectroscopy. These data are in accord with solvent accessibility calculations from three-dimensional models of human renin but differ from findings published previously from similar analysis of mouse submandibular gland renin (Quay, S. C., Heropoulous, A., Commes, K., Dzau, V. J. (1985) J. Biol. Chem. 260, 15055-15058), which is 68% identical in sequence to human renin.
利用离子型淬灭剂和可穿透性淬灭剂测定了重组人肾素三个色氨酸残基的荧光淬灭情况。发现重组人肾素的色氨酸44、200和312完全无法被离子型淬灭剂铯和碘化物接触，而仅部分可被可穿透性淬灭剂丙烯酰胺接触。肾素在325nm处有最大荧光发射峰，通过二阶导数光谱法测定，该峰由三个独立成分组成。这些数据与根据人肾素三维模型进行的溶剂可及性计算结果一致，但与先前发表的对小鼠下颌下腺肾素进行类似分析的结果不同（奎伊，S.C.，赫罗普洛斯，A.，科姆斯，K.，Dzau，V.J.（1985年）《生物化学杂志》260，15055 - 15058），小鼠下颌下腺肾素与人类肾素的序列同源性为68%。
