Luo Yin P, Zhang Li, Wu Xiao Y, Hou Wen S, Chen Lin, Tian Xue L, Wen Hui Z
Annu Int Conf IEEE Eng Med Biol Soc. 2020 Jul;2020:3557-3560. doi: 10.1109/EMBC44109.2020.9175875.
Anodal transcranial direct current stimulation (AtDCS) can improve memory and cognitive dysfunction in patients with Alzheimer's disease (AD), which has been proven in basic and clinical studies. Intervention of AD in preclinical stage is important to prevent progression of AD in the aging society. At the same time, there is increasing evidence that a close link exists between cerebrovascular dysfunction and AD disease. Here we investigated the changes of local cerebral blood microcirculation in preclinical AD mouse model after AtDCS based on the previous studies. Twenty-four 6-month-old male APP/PS1 double transgenic mice were randomly divided into three groups: a model group (AD), a model sham stimulation (ADST) group and a model stimulation group (ATD). Eight 6-month-old male C57 wild-type mice served as a control group (CTL). Mice in the ATD group received 10 AtDCS sessions. Two months after the end of AtDCS in the ATD group, the microcirculation parameters of the frontal cortex of the mice in each group, including cerebral blood flow (CBF), blood flow velocity (Velo), oxygen saturation (SO) and relative hemoglobin content (rHb), were obtained by the non-invasive laser-Doppler spectrophotometry system "Oxygen-to-See (O2C)". The results showed that AtDCS increased CBF, Velo and SO, and reduce rHb in APP/PS1 double transgenic mice at the preclinical stage of AD.Clinical Relevance-This shows the positive effect of AtDCS on preclinical AD in cerebrovascular function, and provides effective basic research facts for AtDCS to intervene and delay the clinical application of AD disease.
阳极经颅直流电刺激（AtDCS）可改善阿尔茨海默病（AD）患者的记忆和认知功能障碍，这已在基础和临床研究中得到证实。在老龄化社会中，对临床前期AD进行干预对于预防AD进展至关重要。同时，越来越多的证据表明脑血管功能障碍与AD疾病之间存在密切联系。在此，基于先前的研究，我们调查了AtDCS对临床前期AD小鼠模型局部脑血微循环的影响。将24只6月龄雄性APP/PS1双转基因小鼠随机分为三组：模型组（AD）、模型假刺激组（ADST）和模型刺激组（ATD）。8只6月龄雄性C57野生型小鼠作为对照组（CTL）。ATD组小鼠接受10次AtDCS治疗。在ATD组AtDCS结束2个月后，通过无创激光多普勒分光光度系统“Oxygen-to-See（O2C）”获取每组小鼠额叶皮质的微循环参数，包括脑血流量（CBF）、血流速度（Velo）、血氧饱和度（SO）和相对血红蛋白含量（rHb）。结果显示，AtDCS可增加临床前期AD的APP/PS1双转基因小鼠的CBF、Velo和SO，并降低rHb。临床意义——这表明AtDCS对临床前期AD的脑血管功能具有积极作用，并为AtDCS干预和延缓AD疾病的临床应用提供了有效的基础研究依据。
