Romaniuk A, Prop J, Petersen A H, Nieuwenhuis P, Wildevuur C R
J Heart Transplant. 1986 Nov-Dec;5(6):455-60.
Expression of class II antigens was investigated in the normal rat lung and in lung allografts during acute rejection and after cyclosporine treatment. Therefore cryostat sections were stained with an immunoperoxidase technique that used anti-class II antigen monoclonal antibodies. In normal lungs only cells of the lymphoid tissue expressed class II antigens. During acute rejection, class II antigens were expressed by infiltrating recipient lymphocytes and were also induced on the graft's bronchial epithelium. After cyclosporine treatment, signs of rejection in the lung were restricted to the bronchial epithelium, again expressing class II antigens. These results suggest that the class II antigen positivity of bronchial epithelium may be used as a specific index for pulmonary rejection when infection as an inducing factor can be excluded. Furthermore, the epithelium, once it has become class II antigen positive, is likely to be a target for acute or low-grade rejection, resulting in the development of bronchiolitis obliterans.
在正常大鼠肺以及急性排斥反应期间和环孢素治疗后的肺同种异体移植物中研究了II类抗原的表达。因此，使用抗II类抗原单克隆抗体的免疫过氧化物酶技术对冷冻切片进行染色。在正常肺中，仅淋巴组织的细胞表达II类抗原。在急性排斥反应期间，浸润的受体淋巴细胞表达II类抗原，并且移植物的支气管上皮也被诱导表达。环孢素治疗后，肺中的排斥反应迹象仅限于支气管上皮，其再次表达II类抗原。这些结果表明，当可以排除感染作为诱导因素时，支气管上皮的II类抗原阳性可用作肺排斥反应的特异性指标。此外，一旦上皮细胞变为II类抗原阳性，它很可能成为急性或低度排斥反应的靶标，导致闭塞性细支气管炎的发展。
