Cardoso Gisele Antoniazzi, Deszo Marina Santos, Torres Tatiana Teixeira
Departamento de Genética e Biologia Evolutiva, Instituto de Biociências, Universidade de São Paulo (USP), São Paulo, SP 05508-090, Brazil.
Departamento de Genética e Biologia Evolutiva, Instituto de Biociências, Universidade de São Paulo (USP), São Paulo, SP 05508-090, Brazil.
Genomics. 2021 Jan;113(1 Pt 2):699-706. doi: 10.1016/j.ygeno.2020.09.066. Epub 2020 Oct 3.
The Oestroidea superfamily is characterized by the diversity of feeding preferences among closely-related species; these flies are saprophagous, obligate parasites, or facultative parasites. We used gene expression and coding sequence data from five species (Cochliomyia hominivorax, Chrysomya megacephala, Lucilia cuprina, Dermatobia hominis, and Oestrus ovis) to identify underlying genetic differences involved in the diverse lifestyles. We tested whether 1287 orthologs have different expression and evolutionary constraints under different scenarios. We found two up-regulated genes; one in species causing cutaneous myiasis that is involved in iron transportation/metabolization (ferritin), and another in species causing traumatic myiasis that responds to reduced oxygen levels (anoxia up-regulated-like). Our evolutionary analysis showed a similar result. In the Co. hominivorax branch, we found one gene with the same function as ferritin that may be evolving under positive selection, spook. This is the first step towards understanding origins and evolution of parasitic strategy diversity in Oestroidea.
狂蝇总科的特点是亲缘关系密切的物种之间取食偏好具有多样性；这些苍蝇是腐食性的、专性寄生虫或兼性寄生虫。我们使用了来自五个物种（嗜人锥蝇、大头金蝇、铜绿蝇、人皮蝇和羊狂蝇）的基因表达和编码序列数据，以确定参与不同生活方式的潜在遗传差异。我们测试了1287个直系同源基因在不同情况下是否具有不同的表达和进化限制。我们发现了两个上调基因；一个在引起皮肤蝇蛆病的物种中，参与铁运输/代谢（铁蛋白），另一个在引起创伤性蝇蛆病的物种中，对氧水平降低作出反应（缺氧上调样）。我们的进化分析显示了类似的结果。在嗜人锥蝇分支中，我们发现一个与铁蛋白功能相同的基因可能在正选择下进化，即幽灵基因。这是了解狂蝇总科寄生策略多样性起源和进化的第一步。
