Wood R D, Hutchinson F
J Mol Biol. 1987 Feb 20;193(4):637-41. doi: 10.1016/0022-2836(87)90346-9.
DNA sequences were determined for 56 mutations induced by ultraviolet light in the lambda cI gene of an Escherichia coli uvr+ lysogen, which should reflect those occurring in the E. coli chromosome. The spectrum of mutagenesis was similar to that found in the cI gene of irradiated phase assayed in uvr- host cells, except that the fraction of transversions is about 35% in prophage and about 15% in phage. The cause of this difference is not known. Of 17 frameshifts in phage and prophage, six have an accompanying base substitution. These double mutational events are consistent with a model in which a photoproduct in a template can cause a DNA polymerase to insert a wrong base and destabilize the next few bases added, thus leading to a frameshift by a slippage mechanism.
测定了紫外线诱导大肠杆菌uvr +溶原菌λcI基因中56个突变的DNA序列，这些突变应反映大肠杆菌染色体中发生的突变。诱变谱与在uvr -宿主细胞中检测的受辐照噬菌体cI基因中的诱变谱相似，只是在原噬菌体中转换的比例约为35%，在噬菌体中约为15%。这种差异的原因尚不清楚。在噬菌体和原噬菌体的17个移码突变中，有6个伴有碱基置换。这些双重突变事件与一个模型一致，即模板中的光产物可导致DNA聚合酶插入错误碱基并使随后添加的几个碱基不稳定，从而通过滑动机制导致移码突变。
