Marino Kaitlyn M, Silva Emani R, Windelborn James A
Department of Biology Washington College Chestertown MD USA.
Department of Psychology Washington College Chestertown MD USA.
Animal Model Exp Med. 2020 Aug 10;3(3):256-263. doi: 10.1002/ame2.12132. eCollection 2020 Sep.
Zebrafish models for neurovascular diseases offer new methods for elucidation of molecular pathways to tissue damage. External fertilization and high fecundity provide opportunities for transgenics and other forms of genetic manipulation that are more accessible than offered by mammalian models of disease. Furthermore, behavioral analyses of zebrafish allow for connection of molecular pathways to organismal outputs such as locomotion, learning, and memory. Unfortunately, a zebrafish model of hypoxia-ischemia has been slow to catch on, possibly due to hypoxia exposure protocols that are challenging to reproduce and result in high mortality.
In this study, we have introduced a predictable and simple method of hypoxia induction, the addition of sodium sulfite to aquarium water. The effects of this treatment on zebrafish locomotion were compared to those of zebrafish exposed to hypoxia induced by nitrogen gas bubbling, a method used in previous reports.
We found that hypoxia induced by sodium sulfite significantly impaired locomotion in the hours following treatment, and its effects did not differ from those caused by nitrogen gas hypoxia.
These results indicate that hypoxia by sodium sulfite represents an effective and easily reproducible method for the study of hypoxia-ischemia in zebrafish.
用于神经血管疾病研究的斑马鱼模型为阐明组织损伤的分子途径提供了新方法。体外受精和高繁殖力为转基因及其他形式的基因操作提供了机会，这些操作比疾病的哺乳动物模型更容易实现。此外，对斑马鱼的行为分析能够将分子途径与诸如运动、学习和记忆等机体表现联系起来。遗憾的是，缺氧缺血的斑马鱼模型一直未得到广泛应用，这可能是由于缺氧暴露方案难以重复且死亡率高。
在本研究中，我们引入了一种可预测且简单的缺氧诱导方法，即在水族箱水中添加亚硫酸钠。将这种处理对斑马鱼运动的影响与暴露于氮气鼓泡诱导的缺氧环境中的斑马鱼（先前报道中使用的方法）进行了比较。
我们发现，亚硫酸钠诱导的缺氧在处理后的数小时内显著损害了斑马鱼的运动，其效果与氮气诱导的缺氧没有差异。
这些结果表明，亚硫酸钠诱导的缺氧是一种用于研究斑马鱼缺氧缺血的有效且易于重复的方法。
