Department of Pathology, Oslo University Hospital, Norwegian Radium Hospital, Oslo, Norway.
Faculty of Medicine, Institute of Clinical Medicine, University of Oslo, Oslo, Norway.
Cytopathology. 2021 Mar;32(2):161-168. doi: 10.1111/cyt.12921. Epub 2020 Nov 20.
To analyse the expression and clinical role of the phosphatase PTPN1 (PTP1B) in serous effusions.
PTPN1 mRNA expression by quantitative RT-PCR was analysed in 83 high-grade serous carcinoma (HGSC) and 15 malignant mesothelioma (MM) effusions. PTP1B and phospho-PTP1B (pPTP1B) protein expression by immunohistochemistry was analysed in 62 HGSC and 44 MM effusions.
PTPN1 mRNA (P = .048), PTP1B protein (P = .047) and pPTP1B protein (P < .001) were overexpressed in HGSC compared to MM effusions. PTPN1 mRNA was additionally overexpressed in post-chemotherapy HGSC effusions compared to chemo-naïve effusions (P = .005). However, pPTP1B protein expression was higher in effusions from patients with FIGO stage III compared to stage IV (P = .006), and higher expressions of both PTPN1 mRNA (P = .041) and PTP1B protein (P = .035) in HGSC effusions were associated with better (complete) chemotherapy response at diagnosis. PTPN1 RNA and protein expression was unrelated to survival in HGSC, whereas a trend for shorter overall survival (P = .06) was found for MM patients whose tumours expressed pPTP1B protein.
PTPN1 is overexpressed in HGSC compared to MM effusions, and may be a marker of better chemotherapy response in the former. Whether PTPN1 activation is informative of adverse outcome in MM merits further investigation.
分析磷酸酶 PTPN1（PTP1B）在浆膜液中的表达及其临床作用。
通过定量 RT-PCR 分析 83 例高级别浆液性癌（HGSC）和 15 例恶性间皮瘤（MM）浆膜液中的 PTPN1mRNA 表达。采用免疫组织化学法分析 62 例 HGSC 和 44 例 MM 浆膜液中的 PTP1B 和磷酸化 PTP1B（pPTP1B）蛋白表达。
与 MM 浆膜液相比，HGSC 浆膜液中 PTPN1mRNA（P=0.048）、PTP1B 蛋白（P=0.047）和 pPTP1B 蛋白（P<0.001）过度表达。与化疗前浆膜液相比，化疗后 HGSC 浆膜液中 PTPN1mRNA 表达进一步上调（P=0.005）。然而，FIGO 分期 III 期患者的浆膜液中 pPTP1B 蛋白表达高于 IV 期患者（P=0.006），且 HGSC 浆膜液中 PTPN1mRNA 和 PTP1B 蛋白表达越高，诊断时完全化疗反应越好（P=0.041 和 P=0.035）。在 HGSC 中，PTPN1RNA 和蛋白表达与生存无关，而 pPTP1B 蛋白表达阳性的 MM 患者总生存时间（P=0.06）有缩短趋势。
与 MM 浆膜液相比，HGSC 浆膜液中 PTPN1 过度表达，可能是前者化疗反应更好的标志物。PTPN1 激活是否对 MM 不良预后有提示作用，值得进一步研究。
