State Key Laboratory of Power Transmission Equipment & System Security and New Technology, School of Electrical Engineering, Chongqing University, Chongqing, China.
State Grid Yangzhou Power Supply Company, Yangzhou, China.
Bioelectromagnetics. 2020 Dec;41(8):617-629. doi: 10.1002/bem.22300. Epub 2020 Oct 7.
Picosecond pulse electrical fields (psPEFs), due to their high temporal-resolution accuracy and localization, were viewed as a potential targeted and noninvasive method for neuromodulation. However, few studies have reported psPEFs regulating neuronal activity in vivo. In this paper, a preliminary study on psPEFs regulating action potentials in hippocampus CA1 of rats in vivo was carried out. By analyzing the neuronal spike firing rate in hippocampus CA1 pre- and post-psPEF stimulation, effects of frequency, duration, and dosimetry of psPEFs were studied. The psPEF used in this study had a pulse width of 500 ps and a field strength of 1 kV/mm, established by 1 kV picosecond voltage pulses. Results showed that the psPEF suppressed spike firing in hippocampal CA1 neurons. The suppression effect was found to be significant except for 10 s, 10 Hz. For short-duration stimulation (10 s), the inhibition rate of spike firing increased with frequency. At longer stimulation durations (1 and 2 min), the inhibition rate increased and decreased alternately as the frequency increased. Despite this, the inhibition rate at high frequencies (5 and 10 kHz) was significantly larger than that at 10 and 100 Hz. A cumulative effect of psPEF on spike firing inhibition was found at low frequencies (10 and 100 Hz), which was saturated when frequency reached 500 Hz or higher. This paper conducts a study on psPEF regulating spike firing in hippocampal CA1 in vivo for the first time and guides subsequent study on psPEF achieving noninvasive neuromodulation. © 2020 Bioelectromagnetics Society.
皮秒脉冲电场（psPEFs）由于其具有高时间分辨率精度和定位能力，被视为一种潜在的靶向和非侵入性的神经调节方法。然而，很少有研究报道过 psPEFs 调节体内神经元活动。本文首次对体内大鼠海马 CA1 区 psPEFs 调节动作电位进行了初步研究。通过分析海马 CA1 区神经元在 psPEF 刺激前后的尖峰放电频率，研究了 psPEF 的频率、持续时间和计量学的影响。本研究中使用的 psPEF 脉宽为 500 ps，场强为 1 kV/mm，由 1 kV 皮秒电压脉冲产生。结果表明，psPEF 抑制海马 CA1 神经元的尖峰放电。除了 10 s、10 Hz 以外，其余情况下抑制效应均显著。对于短持续时间刺激（10 s），尖峰放电抑制率随频率增加而增加。在较长的刺激持续时间（1 和 2 min）下，随着频率的增加，抑制率交替增加和减少。尽管如此，在高频率（5 和 10 kHz）时的抑制率明显大于在 10 和 100 Hz 时的抑制率。在低频（10 和 100 Hz）时发现 psPEF 对尖峰放电抑制有累积效应，当频率达到 500 Hz 或更高时达到饱和。本文首次对体内海马 CA1 区 psPEF 调节尖峰放电进行了研究，为后续研究 psPEF 实现非侵入性神经调节提供了指导。
