Department of Internal Medicine, Universidade Federal do Ceará, Fortaleza, Brazil.
Department of Morphology, Universidade Federal do Ceará, Fortaleza, Brazil.
Connect Tissue Res. 2021 Sep;62(5):597-604. doi: 10.1080/03008207.2020.1825700. Epub 2020 Oct 7.
To determine alterations of chondroitin sulfate (CS) that reflect cartilage damage in an experimental osteoarthritis (OA) model as well as in human OA samples.
Rats were subjected to anterior cruciate ligament transection (ACLT; OA) or a sham procedure and sacrificed 14, 28, or 70 days after ACLT for histopathology and analysis of extracted CS. Cartilage samples from 14 patients undergoing hip or shoulder arthroplasty secondary to OA or fracture (control) were subjected to the same protocol. The CS content (µg/mg dry cartilage) after proteolysis was determined by densitometry, using agarose-gel electrophoresis. Molar mass (MM) and peak MM of CS were determined using high-performance size-exclusion chromatography (HPSEC).
OA and sham joints at 70 d had 24 [22-24] and 3 [1-6] median histopathology scores, respectively (p < 0.001). Relative CS content (77.7 ± 8.3 µg/mg) was significantly increased in OA samples 70 d after ACLT, as compared to sham (53.5 ± 10.0 µg/mg). Peak MM of CS was higher in OA than in sham samples (P < 0.05). Similarly, CS content and peak MM were higher in cartilage from human OA patients, as compared to fracture samples, reproducing experimental data.
Cartilage matrix from experimental and human OA samples has increased in the relative CS content. Increase in the peak MM distinguishes CS of the extracellular matrix of OA from normal cartilage.
在实验性骨关节炎 (OA) 模型以及人类 OA 样本中，确定反映软骨损伤的硫酸软骨素 (CS) 变化。
对大鼠进行前交叉韧带切断术 (ACLT；OA) 或假手术，并在 ACLT 后 14、28 或 70 天处死，进行组织病理学检查，并分析提取的 CS。对因 OA 或骨折而行髋关节或肩关节置换术的 14 例患者的软骨样本进行相同的方案处理。通过琼脂糖凝胶电泳的密度测定法，确定经蛋白水解后 CS 的含量（µg/mg 干软骨）。使用高效体积排阻色谱法 (HPSEC) 确定 CS 的摩尔质量 (MM) 和峰值 MM。
OA 和 sham 关节在 70 天的组织病理学评分中位数分别为 24 [22-24] 和 3 [1-6]（p<0.001）。OA 样本中 CS 的相对含量（77.7±8.3µg/mg）明显高于 sham 样本（53.5±10.0µg/mg）（p<0.001）。OA 样本中的 CS 峰值 MM 高于 sham 样本（P<0.05）。同样，与骨折样本相比，来自人类 OA 患者的软骨中 CS 含量和峰值 MM 更高，再现了实验数据。
实验和人类 OA 样本的软骨基质中 CS 的相对含量增加。CS 峰值 MM 的增加可将 OA 细胞外基质中的 CS 与正常软骨区分开来。
