Department of Oral and Maxillofacial Surgery, The First Affiliated Hospital of Wenzhou Medical University.
Dent Mater J. 2020 Dec 3;39(6):1072-1079. doi: 10.4012/dmj.2019-249. Epub 2020 Oct 7.
The aim of this study was to evaluate the effects of a collagen/hyaluronic acid coating without or with incorporated heterodimeric bone morphogenetic protein 2/7 (BMP2/7) on in-vitro osteoblastic differentiation on titanium discs. The multilayer collagen/hyaluronic acid coatings without or without incorporated BMP2/7 were deposited on titanium discs via a layer-by-layer technique. The effects of the coatings were evaluated by assessing the alkaline phosphatase (ALP) activity (an early osteoblastic differentiation marker) and the osteocalcin expression (a late osteoblastic differentiation marker). The expression levels of the osteoblastic genes, such as alkaline phosphatase 2 (AKP2) and osteocalcin (OC) were detected using real-time RT-PCR. ALP activity and OC expression were significantly increased when cells were cultured with collagen/hyaluronic acid+BMP2/7 heterodimer (p<0.05). The same result was found in cells with the expression of a BMP2/7 fusion gene, OC and AKP2. These results indicated that collagen/hyaluronic acid+BMP2/7 heterodimer-coated discs might have the potential to greatly enhance osseointegration than a either BMP2 or BMP7 solution or a mixture of BMP2 and BMP7 BMP2/7.
本研究旨在评估胶原/透明质酸涂层（有无异源二聚体骨形态发生蛋白 2/7（BMP2/7））对钛盘上体外成骨细胞分化的影响。通过层层技术将多层胶原/透明质酸涂层（有无异源二聚体 BMP2/7）沉积在钛盘上。通过评估碱性磷酸酶（ALP）活性（成骨细胞分化的早期标志物）和骨钙蛋白表达（成骨细胞分化的晚期标志物）来评估涂层的效果。使用实时 RT-PCR 检测碱性磷酸酶 2 (AKP2)和骨钙蛋白 (OC)等成骨细胞基因的表达水平。当细胞与胶原/透明质酸+BMP2/7 异源二聚体（p<0.05）共培养时，ALP 活性和 OC 表达显著增加。在表达 BMP2/7 融合基因的细胞中也发现了同样的结果，OC 和 AKP2。这些结果表明，胶原/透明质酸+BMP2/7 异源二聚体涂层的盘子可能比 BMP2 或 BMP7 溶液或 BMP2 和 BMP7 的混合物具有更大的增强骨整合的潜力。
