National Institute for Cellular Biotechnology and SSPC-SFI Centre for Pharmaceuticals, Dublin City University, Dublin 9, Ireland.
Metallomics. 2020 Nov 1;12(11):1729-1734. doi: 10.1039/d0mt00146e. Epub 2020 Oct 8.
The modern world has seen exposure of bacterial communities to toxic metals at selective levels. This manifests itself both intentionally, through medicines and un-intentionally through waste streams. There is growing concern that selective exposure to metals may be linked to microbial resistance to antibiotics. For a microbe to become resistant to a specific metal it must first come in contact with it. The transition metal copper has the ability to enter bacterial cells without need for a copper specific uptake mechanism. Copper is commonly used as an antimicrobial in the healthcare industry, consumer products and as a growth promoter of livestock in the agricultural sector. Here we report a study into the uptake of different organic and inorganic sources of copper. A whole-cell bacterial biosensor was developed to quantify the specific uptake of copper from various sources. Furthermore, a cell-free sensor was utilized to investigate the response to copper sources when uptake is eliminated as a factor. The data within suggest inorganic copper to have greatly reduced uptake compared to organic sources and that there is significant difference between copper oxides, CuO and CuO.
现代世界已经看到了细菌群落暴露于有毒金属的选择性水平。这种情况既可以通过药物有意地发生，也可以通过废物排放无意地发生。人们越来越担心，选择性暴露于金属可能与微生物对抗生素的耐药性有关。为了使微生物对特定金属产生抗性，它必须首先接触到它。过渡金属铜具有无需特定铜摄取机制即可进入细菌细胞的能力。铜在医疗保健行业、消费品以及农业部门中作为牲畜的生长促进剂被广泛用作抗菌剂。在这里，我们报告了一项关于不同有机和无机铜来源摄取的研究。开发了一种全细胞细菌生物传感器来定量测定各种来源的铜的特定摄取量。此外，还利用无细胞传感器来研究在摄取被消除为一个因素时对铜源的反应。数据表明，与有机来源相比，无机铜的摄取量大大减少，氧化铜和氧化亚铜之间存在显著差异。
