Department of Cardio-Thoracic Surgery, Yancheng Third People's Hospital (The Affiliated Yancheng Hospital of Southeast University Medical College), Yancheng, Jiangsu, China.
Technol Cancer Res Treat. 2020 Jan-Dec;19:1533033820951817. doi: 10.1177/1533033820951817.
Non-small cell lung carcinoma (NSCLC) is a common malignant tumor with poor prognosis. CircRNA-100876 has been considered to be involved in NSCLC. However, the mechanism by which circRNA_100876 mediated the progression of NSCLC remains unclear.
CCK8 assay and immunofluorescence were used to detect cell proliferation. Flow cytometry and transwell assay were performed to analyze cell apoptosis, migration and invasion, respectively. Verification of possible target for circRNA_100876 and related miR-636 were done using luciferase assay. In addition, western blot was performed to detect the protein expressions in NSCLC cells.
Silencing of circRNA_100876 notably inhibited the proliferation of NSCLC cells. Moreover, downregulation of circRNA_100876 significantly induce the apoptosis of NSCLC cells via mediation of apoptosis-related proteins. In addition, silencing of circRNA_100876 significantly inhibited migration and invasion of NSCLC cells. MiR-636 was the downstream target of circRNA_100876. Meanwhile, RET was the direct target of miR-636. Finally, circRNA_100876 shRNA2 notably suppressed the progression of NSCLC through PI3K/Akt signaling.
CircRNA_100876 knockdown notably suppressed the progression of NSCLC through regulation of miR-636/RET axis, which may serve as a potential target for treatment of NSCLC.
非小细胞肺癌（NSCLC）是一种预后较差的常见恶性肿瘤。CircRNA-100876 已被认为参与了 NSCLC 的发生。然而，circRNA_100876 介导 NSCLC 进展的机制尚不清楚。
CCK8 检测和免疫荧光法检测细胞增殖，流式细胞术和 Transwell 检测分别分析细胞凋亡、迁移和侵袭，利用荧光素酶报告基因实验验证 circRNA_100876 及其相关 miR-636 的可能靶基因，Western blot 检测 NSCLC 细胞中的蛋白表达。
沉默 circRNA_100876 显著抑制 NSCLC 细胞的增殖。此外，circRNA_100876 的下调通过调节凋亡相关蛋白显著诱导 NSCLC 细胞的凋亡。此外，沉默 circRNA_100876 显著抑制 NSCLC 细胞的迁移和侵袭。miR-636 是 circRNA_100876 的下游靶点。同时，RET 是 miR-636 的直接靶标。最后，circRNA_100876 shRNA2 通过 PI3K/Akt 信号通路显著抑制 NSCLC 的进展。
circRNA_100876 的敲低通过调节 miR-636/RET 轴显著抑制 NSCLC 的进展，这可能为 NSCLC 的治疗提供新的靶点。
