Reproduction Group, School of Medicine, University of Antioquia, Calle 70 # 52-21, 050010 Medellín, Colombia.
Reproduction Group, School of Medicine, University of Antioquia, Calle 70 # 52-21, 050010 Medellín, Colombia; Department of Physiology, School of Medicine, University of Antioquia, Calle 70 # 52-21, 050010 Medellín, Colombia.
J Reprod Immunol. 2020 Nov;142:103212. doi: 10.1016/j.jri.2020.103212. Epub 2020 Sep 26.
New evidence suggests that glycan expression in placental cells of women with invasive disorders of pregnancy differs from that in normal pregnant women. Hypothesizing that modifications of glycan expression could account for the course of preeclampsia, we established placental villous histocultures and compared glycan expression in women with preeclampsia with that in normal pregnant women and also in syncytialized BeWo cells, and we tested the effect of glycan expression on the functional phenotypes of circulating natural killer (NK) cells. Histocultures of third-trimester placentae from women with preeclampsia and full-term placentae from healthy pregnant women and BeWo choriocarcinoma cells were assessed for the expression of terminal glycans by lectin-binding assays. Circulating NK cells from nonpregnant healthy donors were tested in vitro for their cytotoxic activity and intracellular cytokine content. Histocultures from women with preeclampsia expressed significantly more mannose than did those from healthy pregnant women. Both histocultures and BeWo cells expressed terminal fucose, mannose, sialic acid, and N -acetylgalactosamine, although mean fluorescence intensity (MFI) expression was lower in choriocarcinoma cells than in cells from histocultures. Cocultures of circulating NK cells with K562 target cells resulted in a dose-dependent cytotoxicity effect, but the use of BeWo cells as target reduced cytotoxic activity; this reduction was not affected by syncytialization. Histocultures of placental villous tissue of women with preeclampsia expressed high levels of terminal mannose. We proposethat placental glycans may modulate the functional activity of circulating NK cells in the context of systemic inflammatory response in preeclampsia.
新证据表明，患有侵袭性妊娠疾病的女性胎盘细胞中的聚糖表达与正常孕妇不同。我们假设聚糖表达的修饰可能导致子痫前期的发生，因此建立了胎盘绒毛组织培养物，并比较了子痫前期患者、正常孕妇和合体化 BeWo 细胞中的聚糖表达情况，并测试了聚糖表达对循环自然杀伤 (NK) 细胞功能表型的影响。通过凝集素结合试验评估了子痫前期患者和健康孕妇足月胎盘以及 BeWo 绒毛膜癌细胞的第三孕期胎盘组织培养物中末端糖的表达。从非妊娠健康供体的循环 NK 细胞在体外测试其细胞毒性活性和细胞内细胞因子含量。与健康孕妇相比，子痫前期患者的组织培养物表达的甘露糖明显更多。组织培养物和 BeWo 细胞都表达末端岩藻糖、甘露糖、唾液酸和 N-乙酰半乳糖胺，尽管癌细胞系中的平均荧光强度 (MFI) 表达低于组织培养物中的表达。循环 NK 细胞与 K562 靶细胞的共培养导致剂量依赖性的细胞毒性效应，但使用 BeWo 细胞作为靶细胞会降低细胞毒性活性；这种降低不受合胞体化的影响。子痫前期患者胎盘绒毛组织的组织培养物表达高水平的末端甘露糖。我们提出，胎盘聚糖可能在子痫前期全身炎症反应的背景下调节循环 NK 细胞的功能活性。
