State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangzhou 510642, China; Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, College of Forestry and Landscape Architectures, South China Agricultural University, Guangzhou 510642, China.
State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi Research Center for Microbial and Enzyme Engineering Technology, College of Life Science and Technology, Guangxi University, Nanning 530004, China.
Int J Biol Macromol. 2020 Dec 15;165(Pt A):1119-1128. doi: 10.1016/j.ijbiomac.2020.09.242. Epub 2020 Oct 6.
Hemicelluloses are effective renewable biopolymers that can be used in many different industrial processes and preparations. In plants, the content of hemicellulose might change with different developmental stages and/or tissues. Thus, in here chemical and structural differences in hemicellulose isolated from the apical, middle and basal segments of sugarcane stem were characterized using chemical techniques. Further, difference in expression levels of genes related to synthesis of hemicelluloses from these three segments were studied by RNA-seq and qRT-PCR etc. The sugarcane hemicellulose backbone was xylose residues connected via β-1,4 glycosidic linkages which was further substituted with arabinose, acetyl and glucuronic acid side chains. Hemicellulose content was higher in the middle and basal segments with less backbone substitutions compared to apical segments. In terms of gene expression, hemicellulose synthesis and modification genes were intensely expressed in middle and basal segments. Taken together, our research describes differences in hemicellulose content and substitutions in sugarcane during xylogenesis, which will increase our knowledge for finding more refined use of sugarcane bagasse.
半纤维素是有效的可再生生物聚合物，可用于许多不同的工业过程和制剂。在植物中，半纤维素的含量可能会随着不同的发育阶段和/或组织而变化。因此，在这里使用化学技术对从甘蔗茎的顶端、中部和基部分离出的半纤维素的化学和结构差异进行了表征。此外，通过 RNA-seq 和 qRT-PCR 等方法研究了这三个部位与半纤维素合成相关的基因表达水平的差异。甘蔗半纤维素主链由通过β-1,4 糖苷键连接的木糖残基组成，这些残基进一步被阿拉伯糖、乙酰基和葡萄糖醛酸侧链取代。与顶端部分相比，中间和基部的半纤维素含量更高，且主链取代较少。就基因表达而言，半纤维素合成和修饰基因在中部和基部强烈表达。总之，我们的研究描述了在木质素形成过程中甘蔗中半纤维素含量和取代的差异，这将增加我们对半纤维素的了解，从而更精细地利用甘蔗渣。
