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生物胺对凡纳滨对虾血细胞免疫应答及免疫调控机制的影响。

Effects of biogenic amines on the immune response and immunoregulation mechanism in hemocytes of Litopenaeus vannamei in vitro.

机构信息

The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao, 266003, PR China.

The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao, 266003, PR China.

出版信息

Mol Immunol. 2020 Dec;128:1-9. doi: 10.1016/j.molimm.2020.09.021. Epub 2020 Oct 6.

Abstract

The effects of three biogenic amines (DA, NE and 5-HT) on the immune signaling pathway and immune response of hemocytes in shrimp were investigated through in vitro experiments. The results showed that the G protein effectors (AC, PLC), the second messengers (cAMP, DAG), Calmodulin (CaM) and protein kinases (PKA, PKC) of DA and NE groups shared a similar trend in which all intracellular signaling factors increased significantly and reached the maximum at 3 h. The concentrations of AC, cAMP and PKA in 5-HT groups decreased significantly compared with the control group, while the concentrations of PLC, CaM, DAG and PKC in 5-HT groups increased markedly. The immune parameters such as total hemocyte count (THC), cell viability, antibacterial activity and bacteriolytic activity, as well as prophenoloxidase (proPO) activity in three biogenic amines groups decreased significantly, while the phenoloxidase (PO) activity increased significantly. The phagocytic activity in DA and NE groups decreased significantly, while that in 5-HT groups increased markedly and reached the highest level at 1 h. Among these three biogenic amines, DA showed the strongest effect on the immune activity of the hemocytes, whereas 5-HT had the least effect. In addition, we speculated that DA and NE might regulate phagocytosis by activating intracellular AC-cAMP-PKA pathway while 5-HT might inhibit intracellular AC-cAMP-PKA pathway. Moreover, the activation of proPO system might be related to PLC-DAG-PKC and PLC-CaM pathway.

摘要

采用体外实验的方法研究了三种生物胺(DA、NE 和 5-HT)对虾血细胞免疫信号通路和免疫应答的影响。结果表明,DA 和 NE 组的 G 蛋白效应器(AC、PLC)、第二信使(cAMP、DAG)、钙调蛋白(CaM)和蛋白激酶(PKA、PKC)的所有细胞内信号因子均显著增加,在 3 h 时达到最大值。与对照组相比,5-HT 组的 AC、cAMP 和 PKA 浓度显著降低,而 PLC、CaM、DAG 和 PKC 浓度明显升高。三种生物胺组的总血细胞计数(THC)、细胞活力、抗菌活性和溶菌活性以及酚氧化酶原(proPO)活性均显著降低,而过氧化氢酶(PO)活性显著升高。DA 和 NE 组的吞噬活性显著降低,而 5-HT 组的吞噬活性显著升高,在 1 h 时达到最高水平。在这三种生物胺中,DA 对血细胞的免疫活性影响最大,而 5-HT 的影响最小。此外,我们推测 DA 和 NE 可能通过激活细胞内 AC-cAMP-PKA 通路来调节吞噬作用,而 5-HT 可能抑制细胞内 AC-cAMP-PKA 通路。此外,PO 系统的激活可能与 PLC-DAG-PKC 和 PLC-CaM 通路有关。

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