Evaluation of flocculation performance of amphoteric flocculant when harvesting microalgae Coccomyxa sp. KJ by response surface methodology.

A response surface methodology was used to investigate the flocculation performance of an amphoteric flocculant (acrylamide-methacrylic acid ester-acrylic acid copolymer [ACPAM]) for harvesting microalgae. After three potential influencing factors (pH, dosage, and the stirring speed of an intensive mixing step ω) passed screening in experiments using a Plackett-Burman design, steepest ascent experiments were conducted to identify the parameters for Box-Behnken assessments. In those assessments, ω, dosage, ω, dosage, and ω ∙ dosage were identified as significant factors. This model was optimized by removing nonsignificant factors and applying Box-Cox transformation, both of which significantly improved the adequacy of the model. An optimized set of conditions (pH = 9.0, ω = 339.3 rpm, and dosage = 28.54 mg/L) was obtained under which flocculation efficiency (FE) was predicted to be 95.85% and 98.00% for the nonsignificant factors removed and Box-Cox transformed models, respectively, compared to an experimentally determined value of 98.06%. Thermal stability analyses showed that the ACPAM was generally stable below 100 °C with some weight loss caused by moisture evaporation. However, crosslinking of its molecules by imidization and condensation started to occur at 120 °C, resulting in a lower flocculation performance. Finally, the applicability of the ACPAM was studied by comparing its FE to those of two other flocculants (AlCl and chitosan) when harvesting three microalgal species. The results showed flocculation performance of ACPAM varied with microalgae species, for one species the ACPAM dosage needed was highest while for another species, the dosage was lowest.