Srisrattakarn Arpasiri, Tippayawat Patcharaporn, Chanawong Aroonwadee, Tavichakorntrakool Ratree, Daduang Jureerut, Wonglakorn Lumyai, Lulitanond Aroonlug
Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand.
Clinical Microbiology Unit, Srinagarind Hospital, Khon Kaen University, Khon Kaen 40002, Thailand.
Diagnostics (Basel). 2020 Oct 7;10(10):794. doi: 10.3390/diagnostics10100794.
Bloodstream infection (BSI) is a major cause of mortality in hospitalized patients worldwide. is one of the most common pathogens found in BSI. The conventional workflow is time consuming. Therefore, we developed a lateral flow immunoassay (LFIA) for rapid detection of -protein A in positive blood culture samples. A total of 90 clinical isolates including 58 and 32 non- were spiked in simulated blood samples. The antigens were extracted by a simple boiling method and diluted before being tested using the developed LFIA strips. The results were readable by naked eye within 15 min. The sensitivity of the developed LFIA was 87.9% (51/58) and the specificity was 93.8% (30/32). When bacterial colonies were used in the test, the LFIA provided higher sensitivity and specificity (94.8% and 100%, respectively). The detection limit of the LFIA was 10 CFU/mL. Initial evaluation of the LFIA in 20 positive blood culture bottles from hospitals showed 95% agreement with the routine methods. The LFIA is a rapid, simple and highly sensitive method. No sophisticated equipment is required. It has potential for routine detection particularly in low resource settings, contributing an early diagnosis that facilitates effective treatment and reduces disease progression.
血流感染（BSI）是全球住院患者死亡的主要原因。 是在血流感染中发现的最常见病原体之一。传统的工作流程很耗时。因此，我们开发了一种侧向流动免疫分析（LFIA）方法，用于快速检测阳性血培养样本中的 -蛋白A。总共90株临床分离株，包括58株 和32株非 ，被接种到模拟血液样本中。通过简单的煮沸方法提取抗原，并在使用开发的LFIA试纸条进行测试之前进行稀释。结果在15分钟内即可用肉眼读取。所开发的LFIA的灵敏度为87.9%（51/58），特异性为93.8%（30/32）。当使用细菌菌落进行测试时，LFIA具有更高的灵敏度和特异性（分别为94.8%和100%）。LFIA的检测限为10 CFU/mL。对来自医院的20个阳性血培养瓶进行LFIA的初步评估显示，与常规方法的一致性为95%。LFIA是一种快速、简单且高度灵敏的方法。无需复杂的设备。它具有在常规检测中应用的潜力，特别是在资源匮乏的环境中，有助于早期诊断，从而促进有效治疗并减少疾病进展。
