Laboratory of Exercise Physiology, Faculty of Physical Education, University of Campinas (UNICAMP), Av. ÉricoVeríssimo, 701, Campinas, São Paulo, Brazil.
Department of Physical Education, State University of Maringa, Maringa, Brazil.
Sci Rep. 2020 Oct 9;10(1):16880. doi: 10.1038/s41598-020-73943-y.
Exercise training and a healthy diet are the main non-pharmacological strategies for treating chronic conditions, such as obesity and insulin resistance (IR), in adolescents. However, the isolated metabolic changes caused by exercise training without dietary intervention have not yet been established. We investigated how combined training (CT) without dietary intervention altered the concentrations of serum metabolites, biochemical, anthropometric and functional parameters in overweight and obese adolescents. Thirty-seven adolescents (14.6 ± 1.05 years), of both sexes, were randomly assigned to the control group (CG, n = 19) or the training group (TG, n = 18). The CT was composed by resistance training and aerobic training performed in the same session (~ 60 min), three times a week, for 12 weeks. All assessments were performed pre and post-intervention. Metabolomics analyses were conducted using nuclear magnetic resonance spectroscopy (H NMR) in a 600 MHz spectrometer. There was a decrease in body weight (BW), body mass index (BMI), waist circumference (WC), % body fat (%BF), fasting glucose, insulin levels, and insulin resistance (IR), by HOMA-IR, in the TG. An increase in fat-free mass (FFM) was also observed in the CG. The metabolic changes were given mainly by changes in the levels of metabolites 2-oxoisocaproate (↓TG), 3-hydroxyisobutyrate (↑CG and ↓TG), glucose (↓TG), glutamine (↓CG and ↑TG) and pyruvate (↓TG). These findings demonstrate the positive effects of CT program without dietary intervention on metabolomic profile, body composition, biochemical markers, and glucose metabolism in overweight and obese adolescents.
运动训练和健康饮食是治疗青少年肥胖和胰岛素抵抗（IR）等慢性疾病的主要非药物策略。然而，运动训练而不进行饮食干预所引起的孤立代谢变化尚未得到证实。我们研究了在不进行饮食干预的情况下，联合训练（CT）如何改变超重和肥胖青少年的血清代谢物浓度、生化、人体测量和功能参数。37 名青少年（14.6±1.05 岁），男女不限，随机分为对照组（CG，n=19）或训练组（TG，n=18）。CT 由在同一时间（~60 分钟）进行的阻力训练和有氧运动组成，每周三次，共 12 周。所有评估均在干预前后进行。代谢组学分析使用核磁共振波谱（H NMR）在 600 MHz 光谱仪上进行。TG 组体重（BW）、体重指数（BMI）、腰围（WC）、体脂肪百分比（%BF）、空腹血糖、胰岛素水平和胰岛素抵抗（IR）通过 HOMA-IR 均有下降。CG 组的去脂体重（FFM）也有所增加。代谢变化主要是由于代谢物 2-氧代异己酸（↓TG）、3-羟基异丁酸（↑CG 和 ↓TG）、葡萄糖（↓TG）、谷氨酰胺（↓CG 和 ↑TG）和丙酮酸（↓TG）水平的变化所致。这些发现表明，在不进行饮食干预的情况下，CT 方案对超重和肥胖青少年的代谢组学特征、身体成分、生化标志物和葡萄糖代谢有积极影响。
