Shaanxi Key Laboratory of 'Qiyao' Resources and Antitumor Activities, School of Pharmacy, Xi'an Jiaotong University.
The College of life sciences, Northwest University.
Anticancer Drugs. 2021 Mar 1;32(3):306-313. doi: 10.1097/CAD.0000000000001004.
Breast cancer is one of the most common cancers endangering women's health. SRY-related high-mobility-group box 6 (SOX6) is associated with many cancers, though its role has not been reported in breast cancer. Here, we aimed to explore the expression and function of SOX6 in breast cancer. On the basis of the analysis of SOX6 in The Cancer Genome Atlas, Cancer Cell Line Encyclopedia and Genotype-Tissue Expression databases, we revealed that SOX6 was downregulated in breast cancer, and we verified the results at the cellular level by means of western blotting and quantitative real-time PCR. When SOX6 was overexpressed, the proliferation of breast cancer cells was inhibited, and apoptosis was promoted. Moreover, the methylation level of the SOX6 promoter in breast cancer was significantly higher than that in normal tissues. 5'-Aza-2'-deoxycytidine reversed the high level of methylation that was caused by decreased expression of SOX6. This evidence suggests that SOX6 is a tumor suppressor gene associated with breast cancer. This study could provide a new target for breast cancer treatment.
乳腺癌是危害女性健康的最常见癌症之一。与许多癌症相关的性决定区 Y 相关高迁移率族盒 6（SOX6），尽管其在乳腺癌中的作用尚未有报道。在这里，我们旨在探索 SOX6 在乳腺癌中的表达和功能。基于对癌症基因组图谱、癌症细胞系百科全书和基因型组织表达数据库中 SOX6 的分析，我们揭示了 SOX6 在乳腺癌中下调，我们通过蛋白质印迹和实时定量 PCR 在细胞水平上验证了这一结果。当 SOX6 过表达时，乳腺癌细胞的增殖受到抑制，细胞凋亡被促进。此外，乳腺癌中 SOX6 启动子的甲基化水平明显高于正常组织。5'-氮杂-2'-脱氧胞苷逆转了 SOX6 表达下调导致的高甲基化水平。这一证据表明 SOX6 是与乳腺癌相关的肿瘤抑制基因。本研究可为乳腺癌的治疗提供新的靶点。
